CompetitiveRTPCRStrategyforQuantitativeEvaluation3
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantitative RT-PCR by competition validated above. Liver RNA of three different tilapias was assayed in the presence of different quantities of competitor RNA. Densitometry data derived from band intensities of competitor and target for each tilapia were plotted as described i......阅读全文
Competitive RT-PCR Strategy for Quantitative Evaluation -1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I Quantiza
Competitive RT-PCR Strategy for Quantitative Evaluation -2
Determination of Accuracy of the Competitive PCR To test the precision of the results obtained with this competitive PCR, five different amounts of
Competitive RT-PCR Strategy for Quantitative Evaluation -3
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
Competitive RT-PCR Strategy for Quantitative Evaluation -4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
Competitive RT-PCR Strategy for Quantitative Evaluation -5
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of target in
METTLER TOLEDO Wins Frost & Sullivan Award For Competitive Strategy
METTLER TOLEDO Wins Frost & Sullivan AwardFor Competitive Strategy Innovation and LeadershipFrost & Sullivan has presented their 2014 Global Award f
Protocol for competitive RT-PCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior
定量RT-PCR （Quantitative RT-PCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of mRNA expres
半定量RT-PCR （Semi-Quantitative RT-PCR ）
RT-PCR AnalysisSolutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-10025 mM MgCl2use at a concentration of 1.5 mMLysis Solutio
反向PCR
主要内容如下：· RT-PCR· Competitive and Quantative RT-PCR· In Situ RT-PCR· RL-PCR· DNA Contamination· RT-PCR