WIKI资讯
WIKI资讯
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone in fish (20–23). The expression level of tiGHR that we obtained for each studied tissue can be organized in decreased order of expression levels as: liver > muscle > brain > heart > gonads > intestine > stomach > spleen (Fig. 6). The highest e......阅读全文
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
主要内容如下：· RT-PCR· Competitive and Quantative
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
NGS 是一种识别和确认未知致病菌的前景广阔的技术，然而其在生物防御和公共健康应用等方面的时效性，却往往因为缺乏快速、有效、可靠的自动DNA样品制备方法而受到限制。为了突破这种限制，Kim 等设计了一种基于流体分布元件的数字微流体(DMF) 平台，使得多子系统模块能够进入自动NGS库样品
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
FL3500双调制叶绿素荧光仪 （新升级型号为FL6000） FL3500双调制叶绿素荧光仪是专门用于对蓝绿藻或绿藻等微藻，叶绿体或类囊体悬浮物，乃至叶片进行光合作用研究的强大科研工具。仪器具备双通道测量控制，可控制测量样品的温度，并配备单翻转光（STF），内置多种可用户自行修改的测量程序
作者：柳建磊,秦进 作者单位：(成都铁路中心医院,四川 成都 610081)【摘要】 目的：建立一种利用MGB-Taqman探针的快速、灵敏、特异、准确定量检测外周血PSMA mRNA的方法。方法：选择PSMA mRNA的保守区域，设计合成引
第五届金属组学国际研讨会会议日程 Scientific Programme Wednesday, 9th Sept 2015 9:00 -21:00 Registration Wednesday, 9th Sept 2015, Ginkgo Hall
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
生物分子发表的代表性文献 QTRAP：同时具有三重四极杆和线性离子阱性能的独一无二的LC/MS/MS系统 QTRAP系统最早在ASMS 2002上，作为第一台商用的线性离子阱发布，是世界上唯一的线性离子阱和三重四极杆的复合
9月3日，顶级学术期刊《自然》（Nature）发布兰州大学110周年校庆专刊，以“The buzz from China’s west”为题介绍兰州大学的历史及发展，以“Blazing a trail”为题发布了对兰州大学校长严纯华的专访，还分别介绍了兰州大学生命科学、大气科学、医学、草业科学、
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
Determination of Accuracy of the Competitive PCRTo test the precision of the results obtained with this competitive PCR, five different amounts of T (
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
实验概要The SuperScript™ III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity end-p
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
芯片毛细管电泳具有进样量少，灵敏度高，分析速度快等特点，非常适合法医DNA-STR的快速检验。毛细管电泳芯片由于尺寸小，可施加较大场强，所以在几秒钟内就可完成对样品的分离，微阵列毛细管电泳芯片可实现高通量检测则成为目前学者研究的热点。2002年Emrich CA等[31]报道的将高通量384孔毛
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
The RT-PCR method can be used not only to detect specific mRNAs but also to semi-quantitate their levels. Thus, one can compare levels of transcripts
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total &nbs
2 目前国内外应用于检测耐药结核杆菌的分子生物学方法随着分子生物学的发展，尤其是PCR技术的问世，检测耐药可以直接从基因入手，这样与传统的结核杆菌药敏试验相比，不仅大大缩短了检测周期，实现了自动化，而且也降低了生物实验室的危险性。耐药检测包括用PCR扩增基因组内携带的耐药性区域，和进行扩增产物的突变
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
实验概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
郝繁运 综述 中国误诊学杂志 2007年 第一期 第五次全国中青年检验医学学术会议论文汇编 摘要：本文主要介绍了酶免疫分析的发展现状，从酶免疫分析标记技术若干环节的技术进步、在方法学上与现代化技术的融合与发展、酶免疫分析技