WIKI资讯
WIKI资讯
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of target in the range of your expected measurements with different dilutions of competitor with replicas for each point. In our experiment, we ensemble:• 104 molecules of T with 5 × 103, 104, 5 × 104, 7.5 × 104, and 105molecules of C, respectively, with three r......阅读全文
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
主要内容如下：· RT-PCR· Competitive and Quantative
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
FL3500双调制叶绿素荧光仪 （新升级型号为FL6000） FL3500双调制叶绿素荧光仪是专门用于对蓝绿藻或绿藻等微藻，叶绿体或类囊体悬浮物，乃至叶片进行光合作用研究的强大科研工具。仪器具备双通道测量控制，可控制测量样品的温度，并配备单翻转光（STF），内置多种可用户自行修改的测量程序
作者：柳建磊,秦进 作者单位：(成都铁路中心医院,四川 成都 610081)【摘要】 目的：建立一种利用MGB-Taqman探针的快速、灵敏、特异、准确定量检测外周血PSMA mRNA的方法。方法：选择PSMA mRNA的保守区域，设计合成引
第五届金属组学国际研讨会会议日程 Scientific Programme Wednesday, 9th Sept 2015 9:00 -21:00 Registration Wednesday, 9th Sept 2015, Ginkgo Hall
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
生物分子发表的代表性文献 QTRAP：同时具有三重四极杆和线性离子阱性能的独一无二的LC/MS/MS系统 QTRAP系统最早在ASMS 2002上，作为第一台商用的线性离子阱发布，是世界上唯一的线性离子阱和三重四极杆的复合
NGS 是一种识别和确认未知致病菌的前景广阔的技术，然而其在生物防御和公共健康应用等方面的时效性，却往往因为缺乏快速、有效、可靠的自动DNA样品制备方法而受到限制。为了突破这种限制，Kim 等设计了一种基于流体分布元件的数字微流体(DMF) 平台，使得多子系统模块能够进入自动NGS库样品
Determination of Accuracy of the Competitive PCRTo test the precision of the results obtained with this competitive PCR, five different amounts of T (
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
The RT-PCR method can be used not only to detect specific mRNAs but also to semi-quantitate their levels. Thus, one can compare levels of transcripts
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
实验概要The SuperScript™ III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity end-p
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
蛋白质组数据处理暨第三届全国生物质谱学术交流会(第三轮通知) 为了积极促进我国蛋白质组学技术发展和应用、数据挖掘和生物质谱的经验交流，由中国生物化学与分子生物学会蛋白质组学专业委员会、中国质谱学会生物质谱专业委员会和中国化学会分析化学委员会主办，北京蛋白质组研究中心、复旦大学和蛋
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total &nbs
芯片毛细管电泳具有进样量少，灵敏度高，分析速度快等特点，非常适合法医DNA-STR的快速检验。毛细管电泳芯片由于尺寸小，可施加较大场强，所以在几秒钟内就可完成对样品的分离，微阵列毛细管电泳芯片可实现高通量检测则成为目前学者研究的热点。2002年Emrich CA等[31]报道的将高通量384孔毛
即将向全球推广的狂犬病实验诊断新方法----实时定量RT-PCR检测法（简称LN34检测法），可能作为狂犬病基本检测诊断方法，补充或取代当前的金标准：DFA检测。动物和人狂犬病的确诊，必须要有实验室检测结果，否则都只能算疑似病例。目前WHO（世界卫生组织）肯定的人和动物狂犬病的死后诊断的金标准，是直
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
FluidFM 测定细胞粘附力的应用随着时间推移，越来越多的学者开始使用FluidFM 技术进行测定细胞粘附力。以下就近五年的具有代表性的应用进行总结。Cohen 等使用FluidFM 技术对MCF7-MCF10A、MCF7-HS5 的细胞粘附力进行了测定，并与以往的文献进行对比，发现其数据与Hos
实验概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon