BenchTopRadioactiveWorkProtocol
Log in radioactive material received and deduct amount of radioactive material used in each experiment in Radioisotope Log Book.Clear your bench top work area and make sure it is clean.Put on your lab coat and protective gloves. (Note that you are not allowed to wear open toed shoes while doing lab work)Put your radiation badge on your lab coat at the height of your work surface area. (If are working with m......阅读全文
miPS建系protocol
实验概要本实验方法介绍了miPS建系的详细操作流程。主要试剂所用试剂盒： 逆转录病毒体系试剂盒 MIPD0-000-001慢病毒体系试剂盒 MIPD-000-02规格：5次 组分名称货号规格数量逆转录病毒体系/慢病毒体系20ul/支5支助转因子10ul1支小鼠iPS细
Marcantonio－Lab－Protocol－Manual
Protein GelPreparing and Running a Protein Gel (7% Polyacrylamide) A. Preparation of Running Gel Solution 1) Add to a 50 ml cylinder:DD-H2O 26 ml30% a
IHC－Protocol－for－Fr...
实验概要The method provides a IHC protocol for free floating brain sections.实验步骤1. Coronal 30-40 µm sections cut on a freezing microtome. Sections colle
Protein－Expression－and－Purification－Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
Simplified－Arabidopsis－Transformation－Protocol
实验概要Our present protocol (Clough and Bent, 1998; modified from Bechtold et al. 1993) is extremely simple. We have found that the MS salts, hormone
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Insect－DNA－Isolation－Protocol
实验概要The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatw
Protocol－for－Protein－Extraction－for－proteomics
Protocol for Protein Extraction10 % w/v TCA/ acetone/ 0.07 % v/v -MercaptoethanolPlant cells are rich in compounds that interfere with the 2DE separat
Xenograft－Tumor－Model－Protocol
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, repla
Blocking－with－immunizing－peptide－protocol
实验概要The method provides a blocking with immunizing peptide protocol.实验原理Non-specific binding of an antibody to proteins other than the antigen can s
Protocol－for－Aortic－Ring－Assay
ProceduresCover a 48-well plate with Matrigel (100μl/well) of and incubate for 30 min at 37°C, 5% CO2.Sacrifice the1-2 month old mice/rats (WT/mutant
RTPCR－PROTOCOL
RT-PCR PROTOCOL材料与方法………………………………………………………… 1．材料 ………………………………………………………1.1 供试用组织(细胞)…………………………………1.2 主要仪器设备………………………………………1.3 主要试剂……………………………………………1.
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wit
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
qPCR－Protocol－for－SNP－Genotyping
实验概要Platinum® qPCR SuperMix for SNP Genotyping is a ready-to-use reaction mix for the amplification and identification of single-nucleotide polymorp
LentiVirus－Protocol
Lenti-Virus Protocol张端午A. For packing virus in 6-well plate: (if in 12-well plate, reduce all to 1/2)1. Mix the following plasmids in a 1.5 ml eppendo
Double－immunofluorescence:－sequential－protocol
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies separately.实验步骤1. Blocking and sequential incubation 1) Fi
stem－cell－culture－protocol
实验概要stem cell culture protocol主要试剂cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Intracellular－Cytokine－Staining－Protocol
实验概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surf
Protocol－for－Construction－of－BAC－Libraries
Protocol for Construction of BAC Libraries The bacterial artificial chromosome cloning (BAC) system is emerging as the system of choice for const
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
The－protocol－for－LIC－by－Exonuclease－III
The protocol for LIC by Exonuclease III梁耀极1. Design the primers with 15-bp overlap;2. Digest the vector by proper restriction enzyme;For getting high
T－cell－Activation－Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spin
Native－chromatin－immunoprecipitation－protocol
实验概要The method is a native chromatin immunoprecipitation protocol.主要试剂1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below).Spin at 8,
标准PCR
What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for thos