IHCProtocolforFr...
实验概要The method provides a IHC protocol for free floating brain sections.实验步骤1. Coronal 30-40 µm sections cut on a freezing microtome. Sections collected into petri dishes containing 1-2ml 0.1M phosphate buffer (PB). Sections can be kept on a shaker at 4oC for several days before commencing the immunocytochemistry. [If left too long, the sections become harder to mount].2. Deactivate endogenous peroxid......阅读全文
IHC－Protocol－for－Fr...
实验概要The method provides a IHC protocol for free floating brain sections.实验步骤1. Coronal 30-40 µm sections cut on a freezing microtome. Sections colle
IHC－增敏实验
实验方法原理 滚动式循环放大（rolling circle amplification；RCA）法是一种能够在恒温条件下，使标记有寡核苷酸探针（与组织细胞内的核苷酸无相关性）的抗体（特异性一抗或第二抗）与组织细胞内的抗原结合后，在 DNA 聚合酶的作用下，加入的寡核苷酸进行循环扩增，产生
IHC石蜡技术
实验概要免疫组织化学 (IHC) 是确定组织切片上是否存在蛋白及其位置的一种方法。尽管这种方法在定量分析时灵敏度较免疫印迹法或 ELISA 等免疫测定方法低，但它能了解整个组织的情况。适用于癌症等疾病发展及治疗情况的评估。因此，从 IHC 获得的信 息结合显微技术提供了一副“宏图”，使来自其它
IHC－frozen－sections...
实验概要The method provides a guideline procedure and tips for staining of frozen sections.实验步骤Frozen sections: Once mounted on APES coated slides, frozen
IHC－实验操作步骤
免疫组化方法（石蜡切片）*重要提示：参考抗体说明书选用合适的抗体稀释液和抗原修复处理方法。IHC 方法：抗原修复缓冲液/抗体稀释液A. 所需溶液和试剂1. 二甲苯2. 无水乙醇（100% 和 95%，变性无水乙醇，组织学级）3. 去离
IHC石蜡技术
实验概要免疫组织化学 (IHC) 是确定组织切片上是否存在蛋白及其位置的一种方法。尽管这种方法在定量分析时灵敏度较免疫印迹法或 ELISA 等免疫测定方法低，但它能了解整个组织的情况。适用于癌症等疾病发展及治疗情况的评估。因此，从 IHC 获得的信 息结合显微技术提供了一副“宏图”，使来自其它
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表达上清用0.05M NaHCO3稀释到100ul铺ELISA板，37度或室温振荡大于1小时。注意一定要做一个GS115空菌株表达上清作为阴性对照，最好还找一个带有histag的蛋白作为阳性对照。2.TPBS洗板3次，方法：倒掉铺板液，倒置于
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
Immunoblot－Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
ELISPOT－protocol
实验概要The procedure below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits have been designed for detection of various cytokines and g
PCR－protocol
PCR reactionProtocol for 50µl reaction - adjust amounts if necessary, for a 20µl reaction use the same volumes of primer and dNTP-mix, but adjust the
RNAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.
Immunoprecipitation－Protocol
实验概要Immunoprecipitation is a procedure by which proteins or peptides that react specifically with an antibody are removed from solution and examined
PCR－RFLP分析技术（Polymerase－Chain－Reaction–Restriction－Fr...
【实验目的】1．熟悉PCR—RFLP分析技术原理及实验步骤。2．掌握琼脂糖凝胶电泳检测方法。3．了解PCR— RFLP在遗传病基因诊断中的作用。【实验原理】聚合酶链式反应（PCR）是模拟体内DNA复制条件在体外酶促合成特异DNA片段的循环反应，可使目的DNA片段得以迅速扩增。其主要步骤是：将待扩增的
图说鲜肉IHC/ICC晋级攻略
西土有一名寺，昂迪·赛司腾姆寺，有大师。一日，来了一位东土小鲜肉。鲜肉问大师：“学业有惑，可解？”大师反问：“你术业可是生物？”鲜肉惊奇：“是，大师神奇，何以得知？”大师指鲜肉鞋履：“鞋上两块蓝斑，乃考马斯亮蓝，其渍尚新，你近来必在捣弄蛋白，对否？”鲜肉羞赧：“正是近日检测蛋白，跑胶无数，不慎滴上。
Phycoerythrin－conjugation－protocol
Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal an
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
cDNA/AFLP－Protocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
Migration－Assay－Protocol
Materials to be prepared beforehand:1) FBS free medium2) 10% FBS medium3) Cell migration filter insert ( Transwell®, 12mm Diameter, 12 μm Pore Size.)P
Protocol－for－Trichl...
实验概要The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication)
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Protocol－of－Northern－blot
Protocol of Northern blot质粒的转化和扩增质粒的鉴定目的基因片段的切割3.1样品双酶切(175μl水解体系)DW 115μlBuffer B(10×) 17.5μlBaM H 15μlPst I 17μlDNA(MMP-9) 16μlBSA 4.5μl37℃水浴，3h。3.2