AsepsisandAsepticPracticesintheOperatingRoom——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,4All sterile items should be dispensed to the sterile field by methods that preserve the integrity of the items and sterile field.1 Nonsterile personnel, usually the circulating nurse, must use good judgement when dispensing sterile items onto the sterile field eit......阅读全文
Amicon－Stirred－Ultrafiltration－Cells
DescriptionFor protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW)
突破！睿创团队中红外带间级联激光器研究取得重要进展
近日，睿创研究院及睿创光子团队在中红外带间级联激光器（Interband cascade laser，ICL）的研究取得重要进展，相关团队实现了高性能、室温连续工作、多个激射波长的带间级联激光器系列，结合分子束外延技术，在InAs衬底上生长带间级联激光器材料，制备的窄脊器件室温激射波长接近4.6
医学微生物学(Medical－Microbiology)词汇归纳（一）
adenovirus 腺病毒 adsorption 吸附aerobe 需氧菌aflatoxin 黄曲霉毒素airborne transmission 空气传播amantadine 金刚烷胺anaerobe 厌氧菌anthrax 炭疽antigenic drift 抗原漂移,抗原转变asepsis 无
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wit
CHROMATOGRAPHICP－EAKS－－色谱峰
13.1. Chromatographic analysis should meet ALCOA+ principles.色谱分析应符合ALCOA+原则。13.2. Factors considered during method validation should include方法验证中考虑的因
Whole－mount－antibody－staining－ofzebrafish－embryos－formarkers－ofsegmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Immunocytochemistry...
实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure 1) Coat coverslips wit
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
ELISA:－Sandwich
实验概要The following protocol provides a method of sandwich ELISA by Peprotech.主要试剂Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liqu
前世三通用型手术室显微镜，我是全科的
前世三通用型手术室显微镜，我是全科的1953年，OPMI 1 (Operating Microscope Number One)问世，虽然这台显微镜是为中耳手术设计的，但仍被认为是通用型显微镜的鼻祖。随后的几年里，通过不断改进，手术显微镜开始在眼科中应用，主要是角膜、巩膜的外眼手术。第一台手术显微镜
竞争性分析Epitope－Mapping实验方法
ABSTRACTThe simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assa
MINICHROMOSOME－MICROTUBULE－BINDING－ASSAY
Determine the OD600 and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105, 1x105, and 3.0x
Preparation－of－Rat－Liver－Cell－Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or flash fro
Preparation－and－Staining－of－Frozen－Tissue－Sections
I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane)Liquid NitrogenDry icePeel-Away?/sup> base moldsFrozen tiss
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
RNA－Purification－from－1020－mg－Paraffinembedded－Tissue
实验概要The E.Z.N.A.® SQ Tissue RNA Kit is designed for isolating total RNA from animal tissue and cultured cells. The solution based system can be easi
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
Chlamydomonas－Fixation－for－Transmission－Electron－Microscopy
Chlamydomonas Fixation for Transmission Electron MicroscopySolutions:Chlamydomonas culture medium + 2% glutaraldehyde (5 ml medium + 0.9 ml 25% glutar
Separation－of－Platelets－from－Whole－Blood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamb
Series－80－Conveyorised－Metal－Detectors
Series 80 Conveyorised Metal Detectors 产品型号：Series 80产品品牌：梅特勒-托利多产品价格：电询 In a competitive market place y
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
Immunofluroescence－Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of
Carbohydrate－Assays
Carbohydrate AssaysREFERENCE: Wright and Rebers, Anal. Biochem. 49: 307-319, 1972.OBJECTIVE: To determine the relative amounts ofLPS carbohydrates pre
Permeabilization－of－gramnegative－bacteria－with－KPi/hexane
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 oC, 200 rpm) Escherichia coli, Enterobacter aerogenes or Pseudomonas aeruginosa cells.Wash
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
E.Z.N.A.®－Plasmid－Maxi－Kit－vacuum－Protocol
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
Preparation－of－tubulin2
DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport