FIXATIONOFPLANTMETAPHASECHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant material; except for ice water, the solution should be the same temperature as that where the plants grow to avoid shock.(i) ice water (for cereals and temperate grasses): put distilled or deionized water in a clean plastic bottle, shake to aerate and keep at –20......阅读全文
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
Metaphase－chromosome－preparation
Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask
Making－Metaphase－Spread
Materials:Colcemid (GIBCO: cat #: 15210-040)Hypotonic solution (pre-warmed 37C): 1:1 0.4% KCl + 0.4% Sodium citrateFixative: 3:1 MeOH + Acet
Cell－Cycles－－Introduction
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
Counting－ES－cell－Chromosomes
(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab)1) Plate cells onto gelatinized plates (35 or 60 mm) witho
In－Situ－Hybridization－to－Somatic－Chromosomes－in－Drosophila
In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. DernburgINTRODUCTIONIn situ hybridization was originally developed as a technique fo
Horizontal－Transfer－of－Supernumerary－Chromosomes－in－Fungi
Several species of filamentous fungi contain so-called dispensable or supernumerary chromosomes. These chromosomes are dispensable for the fungus
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
实验概要Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a mito
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining1
INTRODUCTIONThe formidable size and structure of polytene chromosomes allowmapping of chromosomal protein distributions at very high resolution.This p
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining2
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip. 13. Incubate
杨芃原：Exploration－of－human－chromosomes－by－biomass－spectrometry
复旦大学化学系 杨芃原教授 2014年4月26日，首届全国质谱分析学术研讨会在北京西郊宾馆盛大开幕。来自复旦大学的杨芃原教授带来了题为《Exploration of human chromosomes by biomass spectrometry based
Auxin－Immunolocalization－in－Plant－Tissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g
A－quick－method－to－isolate－plant
Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
PCR－from－Plant－Tissue
1.protocol(1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH(2)put in boiling H2Ofor 30 sec
PCR－from－Plant－Tissue
PCR from Tissue Reference: Klimyuk et.al., 1993, Plant J. 3:493-494 Last updated: 1/27/00 By: Kay Schneitz collect piece of tissue (e.g., piec
细胞组分和细胞器——染色体
Chromosomal DNA Prep : cultured cells/tissue samples (Mike A Dyer)This protocol was developed for cultured cells but should be appropriate for dissoci
李家洋应邀在PLANT－CELL撰写Reflections－on－Plant－Cell－Classics文章
The Plant Cell是植物领域的著名学术期刊，对植物学的发展起到了重要的引领作用。为庆祝创刊30周年，The Plant Cell杂志社邀请部分编委会成员及其他科学家对发表在该杂志的重要研究工作进行评述，以期增进对植物科学过去30年中重要研究进展的认识，激发对未知领域的探索。 李家洋研
CGH－Protocols－（四）
CGH Image acquisitionImages were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss,
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
Chemical－Genomics－Approaches－in－Plant－Biology
Chemical genomics (i.e., genomics-scale chemical genetics) approaches are based on the ability of low-molecular-mass molecules to modify biologica
MS－Plant－Tissue－Culture－Medium
Component mg/l in MS mg/l in stock Amount for
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
Protocol－for－Construction－of－BAC－Libraries
Protocol for Construction of BAC Libraries The bacterial artificial chromosome cloning (BAC) system is emerging as the system of choice for const
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
植物病毒(plant－viruses)RNA提取
大多植物病毒RNA为单链RNA，并且其极性与mRNA极性相同，植物病毒RNA提取较为简单，一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液（10mg/ml）。 二、设备 冷冻台式离心机，低温真空干燥仪，电泳仪，电泳槽。 三、试剂 TE
CGH－Protocols－（三）
Hybridizationreagents: labeled tumor and normal-DNA (see protocol Nick translation) salmon sperm DNA, 10 mg/ml (e.g. Promega) human Cot1 DNA, 1 mg/ml
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to produ