AsepsisandAsepticPracticesintheOperatingRoom——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,4All sterile items should be dispensed to the sterile field by methods that preserve the integrity of the items and sterile field.1 Nonsterile personnel, usually the circulating nurse, must use good judgement when dispensing sterile items onto the sterile field eit......阅读全文
高分辨率太赫兹时域光谱仪系统（0.34－THz）参数
指标参数TeraSys 4000Frequency range0.3-4 THzOutput power>50 nWSpectral resolution100:1, verticalInput voltage110V/240V, 50 or 60 HzPower consumptin< 60WWa
Double－immunofluorescence:－sequential－protocol
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies separately.实验步骤1. Blocking and sequential incubation 1) Fi
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Purification－of－Genomic－DNA－Using－PureLink™－Silica－Columns
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently isolate ge
Quick－and－Easy－Isolation－of－Genomic－DNA－from－Yeast
ProcedureTransfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microcentrif
笔式电导率计的技术指标
主要技术指标 测量范围 Range：0~9999mg/L（ppm） 分辨率 Resolution：1µs/cm 精度：Accuracy：±2%F.S 工作电压 Battery：2*3V（CR2030电池） 工作温度 Operating Temperature： 0℃～50℃ 校正Ca
RNAse－A－Treatment－of－Mouse－Cells
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
SQ－Blood－DNA－Mini－Protocol－for－Buffy－Coat
实验概要The buffy coat fraction of whole blood is enriched with WBC, and usually gives at least 5-fold more DNA than the same volume of blood. To prep
Human－FastTrack－mRNA－Isolation
Preparation of Cells1.Prepare or collect between 2x107 cells for each mRNA prep (will yield about 10-20µg of mRNA). If PBMCs from a whole blood sample
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem
Standard－Protocols－Autoradiography－(35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
Autoradiography－(35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
基因敲除（KO）在验证抗体特异性的应用
抗体作为基础科学研究和临床试验常用的工具之一，主要通过抗原-抗体的反应揭示蛋白质在生命活动中的变化。尽管它们被广泛的使用，但是没有相关标准以确保商业抗体特异性和质量上的一致性。这使得科学界一直存在“重现危机”，浪费了科学家们大量的时间和金钱，妨碍生命科学研究的快速发展。因此，对抗体进行敏感性和特异性
Cellbased－ELISA－for－primary－cells
1. The 96-well micro-plates are pre-coated with extra cellular matrix.2. Primary cells are grown in the 96-well plates. Seed 100μl of 10,000-20,000
蛋白芯片制作与应用（3）－操作流程
一个经典的蛋白芯片操作流程：Experimental Procedures for Protein Microarrays--------------------------------------------------------------------------------Chemicall
Coupling－Antibodies－to－Protein－A－or－G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination).2. mix antibodies with beads and bind at room temperatu
Immunocytochemistry...
实验概要The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a versatile marker for monitoring physiological processes, visualizi
Maxiprep－of－plasmid－DNA－from－E.coli－protocol
Solutions/reagents:LB broth + selective marker50% sterile glycerolTEG(25mM Tris-Cl, 10mM EDTA, 50mM dextrose)20 mg/ml lysozyme10% SDS4M NaOHautoclaved
关于无菌性脑膜脑炎的简介
无菌性脑膜炎(aseptic meningitis)又称浆液性脑膜炎、淋巴细胞性脑膜炎或病毒性脑膜炎，是多种病毒性神经系统感染的常见表现。无菌性脑膜炎的基本概念是指除细菌或真菌以外的致病因子所致的脑膜炎症，主要特征是脑膜刺激症状和胞脊液细胞增多。虽然多数病例系病毒所致，但也可由其他病原体感染或某
细胞培养常规操作
常规操作(主要内容如下)· Aseptic Technique· Culture Vessels· Cell Counting· Primary Culture· Maintenance of Cell Line ·
美国豁免黄曲霉AF36在杏仁与无花果中的残留限量
食品伙伴网讯 据美国联邦公报消息，3月22日美国环保署发布通报，豁免黄曲霉AF36在按照标识说明与良好操作规范使用时，在杏仁与无花果中的残留限量。 美国环保署对黄曲霉AF36开展了毒理学以及暴露风险评估。经过评估，美国环保署认为，黄曲霉AF36无毒性、无致病性、无传染性，对人体健康无影响，
Decontamination－of－Ethidium－Bromide－Solutions－and－Surfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendio
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
常用缓冲液配制－2
PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOHPBS (5x in 500 mls) 20.45 g NaCl 0.465 g KCl 10.142 g Na2HPO4*7 H2O 0
E.Z.N.A.®－Plasmid－Maxi－Kit－Spin－Protocol
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
Amicon－Stirred－Ultrafiltration－Cells
DescriptionFor protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW)