PreparationandStainingofFrozenTissueSections
I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane)Liquid NitrogenDry icePeel-Away?/sup> base moldsFrozen tissue matrix (OCT?/sup> or Cryomatrix?/sup>)Long forcepsNecropsy toolsSuperfrost Plus slidesLabel base mold and partially fill the mold with frozen tissue matrix.Sacrifice animal by prescribed and approved euthanasia techniques.Remove desired tissues, trim and cut......阅读全文
Preparation－and－Staining－of－Frozen－Tissue－Sections
I. Preparation of Frozen Sections for Sectioning Materials neeed: 2-methylbutane (isopentane) Liquid Nitrogen Dry ice Peel-Away?/sup> ba
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
DNA－Extraction－from－Frozen－Tissue－Sections
Tissue collection, storage, microdissection, sectioning: See separate protocol.Tissue handling: Note that all fresh tissue should be handled as BioSaf
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
组织学——显微解剖
Laser Capture Microdissection (LCM)Introduction to LCM (BJMU) Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture M
IHC－frozen－sections...
实验概要 The method provides a guideline procedure and tips for staining of frozen sections. 实验步骤 Frozen sections: Once mounted on APES coated slides,
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
Extraction－of－RNA－from－Frozen－Sections
RNA Extraction from Frozen Tissue Sections Tissue Handling: Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
组织学——染色
Hematoxylin and Eosin Staining of Tissue for LCM (Arcturus) Immunohistochemical Staining (IHC) (Arcturus)For optimal LCM from IHC samples, it is nec
免疫组织化学
· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)· Immunohistochemistry (Tyner lab)This is a
BrdU－Labeling－Protocol
实验概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
ImmunoLaser－Capture－Microdissection
A: Development of Immuno-LCM Limitation of Microdissection Microdissection of routinely stained or unstained frozen sections has been used succe
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
Histochemistry－－Introduction
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re
Immunohistochemistry－Protocol－for－ParaffinEmbedded－Sections
实验概要The following is a general procedure guide for preparation and staining of formalin-fixed, paraffin-embedded tissues using a purified, unconjuga
Immunohistochemistr...
实验概要Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in tissue. The method exploits the princi
Immunohistochemistry－using－AntiGanglioside－Antibodies
Immunohistochemistry using Anti-Ganglioside Antibodies Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medi
Preparing－cells－and...
实验概要 The method provides a protocol and tips for BrdU staining in tissue sections.Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a syntheti
骨组织βgal免疫染色实验技术方法
ß-gal Staining:Reagents:0.1M Phosphate Buffer (pH 7.3):0.1M Sodium Phosphate monobasic115ml0.1M sodium phosphate dibasic385mlTotal Volume500 mlFix sol
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem
组织学——组织制备
· Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like fixation, dehydration, embedment and subs
组织学——包埋技术
Tissue Preparation for Methy Methacrylate EmbeddingMethyl Methacrylate Embedding Protocols (Energy Beam Sciences, Inc.)General protocol for plastic em
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and