PhageTiter
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clones. Large numbers of clones are often needed for construction of gene libraries that represent large populations of molecules. These libraries are also easy to sort through, as lambda phage clones are particularly easy to array spatially at high density. This is called ......阅读全文
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 µg/l tetracycline.Shake at 200 rpm and 37 °C untill the
Phage－DNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli. Incu
烈性噬菌体（virulent－phage）和温和噬菌体（temperate－phage）
噬菌体(bacteriaphage or phage)是病毒的一类，结构很简单，基本上由一个蛋白质外壳包裹着一些核酸组成的。噬菌体的多样性来自于组成其外壳的蛋白质的种类，以及其染色体的类型和结构的不同。（一）烈性噬菌体（ virulent phage）遗传学上应用最广泛的烈性噬菌体是大肠杆菌（ E.
Lambda－Phage－DNA－Quickprep
suspend a single plaque in 1 ml PSBadsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% malto
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is: •
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix 5.8 g of NaCl, 2 g of MgSO4-7H2O, 50 mL of 1M Tris-HCl, pH 7.5, 0.5 mL of 2% gelatin, and dH2O to 1 L (Autocla
Preparation－of－High－Titer－Adenovirus－in－P11－cells
adapted from Cell Biology, A Lab Manual, second edition, volume 1 -Grow up P11 cells in 15 cm plates to 70 – 80% confluence. -Infect cells with a MOI
Lambda（噬菌体）DNA－Miniprep
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Research StationAugust 26, 1993Background :There are many published method
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
cDNA－LIBRARY－SCREENING
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extract, and 1.0 mL of 1M MgSO4. A
Cosmid－Cloning:－Cell－preparation,－DNA－packaging,－and－Cell－Transfection
Cosmid Cloning: Cell preparation, DNA packaging, and Cell TransfectionProtocol taken from Stratagene's Gigapack packaging extracts instruction man
最主流的细胞活力检测方法—CTG(CELL－TITERGLO)发光法简介
细胞增殖检测技术已广泛应用于分子生物学、遗传学、肿瘤生物学、免疫学、药理和药代动力学等研究领域。细胞增殖是指细胞在周期调控因子的作用下，通过DNA复制、RNA转录和蛋白质合成等复杂反应而进行的分裂系列过程。细胞通过分裂的方式增殖，细胞增殖是生物体的重要生命特征 。单细胞生物以细胞分裂的方式产生新
最主流的细胞活力检测方法——CTG(CELL－TITERGLO)发光法简介
细胞增殖检测技术已广泛应用于分子生物学、遗传学、肿瘤生物学、免疫学、药理和药代动力学等研究领域。细胞增殖是指细胞在周期调控因子的作用下，通过DNA复制、RNA转录和蛋白质合成等复杂反应而进行的分裂系列过程。细胞通过分裂的方式增殖，细胞增殖是生物体的重要生命特征 。单细胞生物以细胞分裂的方式产
Preparing－Lambda－DNA
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
荧光偏振法快速准确高通量检测IgG和含Fc段衍生物
荧光偏振法快速、准确、高通量检测IgG和含Fc段衍生物Dr. Carolanne DohertyValitacell, NIBRT, Fosters Avenue, Blackrock, Dublin, Ireland BMG多功能酶标仪可应用于定量IgG的新技术Valita™TITER
噬菌体的生长
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared Streaking Lambda
Lambda噬菌体
· Lambda DNA Preparation (Stanford DNA Sequence & Technology Center)Detailed protocol for lambda DNA preparation with recipes· Isolati
Cesium－Chloride－Purification－of－T7
SummaryCleaner stocks of T7 that concentrates and purifies T7 bacteriophage.ProtocolGrow 100mL of permissive cells to a density of 108 to 109 cells/ml
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
关于温和噬菌体的基本介绍
根据噬菌体和宿主菌的关系，可将噬菌体分为两类：一类噬菌体在宿主菌细胞内迅速增殖，产生许多子代噬菌体，并最终使宿主菌细胞破裂，这类噬菌体被称为烈性噬菌体（ virulent phage）；另一类噬菌体感染宿主菌后不立即增殖，而是将其核酸整合（Integration）到宿主菌染色体中，随宿主核酸的复
温和噬菌体的分类和特点介绍
根据噬菌体和宿主菌的关系，可将噬菌体分为两类：一类噬菌体在宿主菌细胞内迅速增殖，产生许多子代噬菌体，并最终使宿主菌细胞破裂，这类噬菌体被称为烈性噬菌体（ virulent phage）；另一类噬菌体感染宿主菌后不立即增殖，而是将其核酸整合（Integration）到宿主菌染色体中，随宿主核酸的复制而
Screening－a－cDNA－Library
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
高效液相解决方案4大妙招破解抗体分析难题
妙招1：小身材，大智慧——进样器带馏分收集功能的双三元 DGLC——U3000 1 离线方法在抗体开发前期，对样品进行纯化制备，进行亲和色谱分析，将目标抗体馏分收集到进样盘中，然后再进行后续的抗体药的表征工作。 2 在线分析二维色谱PAT技术同时监控产品产量和质量，原本需要2天时间才知道产量和质量（