HowtoprepareMolecularBiologygradeglycogen
OverviewGlycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg).Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology......阅读全文
How－to－prepare－Molecular－Biology－grade－glycogen
OverviewGlycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be p
制备分子生物学级的糖原
Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
浙大，清华发表Nature－Structural－－Molecular－Biology文章
生物通报道：来自浙江大学生命科学研究院，清华大学的研究人员报道了两个前所未有的GspD通道冷冻电镜结构，为理解胰泌素超家族，以及II类分泌系统底物运输的机制提供了一个结构基础。这一研究成果公布在1月9日的Nature Structural & Molecular Biology杂志上。 在革兰
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
中科院学者Nature－Structural－－Molecular－Biology发表最新成果
10月23日，中国科学院生物化学与细胞生物学研究所国家蛋白质科学中心（上海）许琛琦研究组与牛津大学Omer Dushek研究组合作，在Nature Structural & Molecular Biology上在线发表了题为“Dynamic regulation of CD28 conforma
Nature Structural Molecular Biology揭示细菌脂多糖跨膜转运机理
4月10日，《自然-结构与分子生物学》（Nature Structural & Molecular Biology）在线发表了中国科学院生物物理研究所研究员黄亿华课题组的研究论文Structural basis for lipopolysaccharide extraction by ABC t
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
How－to－interrupt－scintillation
Peter Novick Lab, Department of Cell Biology Yale University School of Medicine HOW TO PERFORM AN INTERRUPT OF ANONGOING AUTOCOUNT1). This program int
DNA体外转染试剂_如何准备转染所需的质粒DNA
转染效率受到诸多因素的影响，除了细胞、培养基和载体等影响因素外，另外一项非常重要的因素便是DNA的质量。为了比较不同厂家的转染效率，我们分别从三家不同的供应商购买了质粒制备试剂盒来制备pEGFP-N3质粒DNA，并通过不同的方法将制备的pEGFP-N3质粒转运至NIH-3T3细胞。pEGFP-N3质
How－to－build－a－BAC－library
Introduction The most important aspect of our cloning vectors is that they are based on the E. coli F-factor replicon. It allows for strict
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
RNAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
Functional－Genomics－and－Structural－Biology－in－the－Definition－of－Gene...
By mid-2007, the three-dimensional (3D) structures of some 45,000 proteins have been solved, over a period where the linear structures of millions
How－Progesterone－Initiates－Oocyte－Membrane
Progesterone (Pg) binds to both intracellular iPR and plasma membrane- bound mPR. (Right Top) After binding to Pg, iPR is recruited to the membrane as
How－to－Make－Simple－Solutions－and－Dilutions
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine
Selfcircularization－of－Linear－DNA
In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deionized water or TE buffer (10-35µl).Add:10X ligation buffer 5µl,50% PEG 400
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
How－do－you－synthesize－your－dsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig
The－E.Z.N.A.®－MagBind™－Dye－terminator－Removal－Procedure
实验概要Excess unincorporated, nonradioactive label can cause high background fluorescence in automated sequencing gels. For optimal sequencing results
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
标准PCR
What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for thos
标准PCR
· What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
Molecular－Weight－Marker
Molecular Weight Markerl HindIII Digest Marker SolutionDigest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl)Add 50 µl 10X Tracking DyeBring volume of the dig
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
早期胚胎发育中的单胚胎细胞基因表达（一）
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology1
By: Christopher P. Toseland1 2 , Martin R. Webb1Affiliation(s): (1) MRC National Institute for Medical Research, London, UK(2) Institut für Zelluläre
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
DNA－Molecular－Weight－Markers
DNA Molecular Weight Markers Lambda DNA Hind III DigestFragmentBase Pairs123,31029,41536,55744,36152,32262,02775648125Lambda DNA EcoR I DigestFragment