MolecularAnalysisandResultsDNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number changes throughout the genome in a single hybridization. CGH is based on co-hybridization of differentially labeled tumor and normal DNA to human metaphase chromosomes. In a typical experiment:The tumor DNA is labeled with FITC-dUTP and normal DNA with TexasRed-dU......阅读全文
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
DNA－Molecular－Weight－Markers
DNA Molecular Weight Markers Lambda DNA Hind III DigestFragmentBase Pairs123,31029,41536,55744,36152,32262,02775648125Lambda DNA EcoR I DigestFragment
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
Restriction－Digests－of－High－Molecular－Weight－Yeast－DNA
Purpose:To perform restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion.Time req
High－Molecular－Weight－Yeast－Liquid－DNA－Preparation
Purpose:To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yi
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS2
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role in many physiological
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Glycosphingolipid－analysis
1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
ASMS－2015－安捷伦关注“Powerful－Partnerships,－Inspiring－Results”
2015年5月31 日-6月4日，第63届美国质谱年会(ASMS 2015)将在美国密苏里州圣路易斯会展广场会议中心召开，小编即将搜罗各公司的精彩活动，敬请关注！ 安捷伦科技公司（NYSE：A）日前宣布，将在5月31日-6月4日的ASMS 2015上推出新的实验室解决方案，帮助客户进一步优化科
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells1
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
RESULTS为您带来全新解决方案
RESULTS为您带来全新解决方案 RESULTS，为Fisher Scientific开创的全新理念，旨在为细胞生物学、蛋白质研究、基因组学和色谱应用等研究领域提供从样品收集到数据采集的完整解决方案。 您的实验室中每天开展的研究工作全都依赖于一样东西……result
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in huma
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity ChromatographyAnalysis of Oligosaccharide Ligands by High Performance Liquid A
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cyt
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first of
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
酵母遗传学技术
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analysis u
第八届全国微全分析系统学术会议微纳米生物分析专场
2013年5月16日-19日，由中国化学会主办、厦门大学承办、复旦大学、浙江大学协办的为期四天的第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议在美丽的海滨城市厦门隆重召开。以下是
Molecular－Weight－Marker
Molecular Weight Markerl HindIII Digest Marker SolutionDigest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl)Add 50 µl 10X Tracking DyeBring volume of the dig
基于epMotion－5075t系统与KPPA－HyperPlus试剂盒的全自动测序...2
Results and DiscussionThe post-ligation qPCR results were used to calculate the percentage of starting material that was successfully adapter ligate
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
对28种癌症研究证实：致癌突变男女有别－|－论文推荐
近日，公开发布于美国冷泉港实验室生物预印本数据库（CSH bioRxiv）中的一项研究首次全面证实了致癌突变的性别差异。 来自加拿大安大略癌症研究所（Ontario Institute for Cancer Research）和美国加州大学洛杉矶分校（University of Califor
用Fluidigm－digital－array芯片研究癌细胞
Technology Review网站在2009年9月30日发表了题为《Analyzing Cancer Cells to Choose Treatments—— Microfluidics chips allow scientists to study circulating cancer
Methylation－Specific－PCR
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam