DecontaminationofEthidiumBromideSolutionsandSurfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendioxide, a toxic gas, when initially mixed.Laboratory Safety Practices and Equipment:-Prepare decontamination solution in the fume hood.-Wear two layers of gloves, lab coat and safety glasses.-Turn off electrical equipment before decontamination.Preparation of Decontaminat......阅读全文
Native-chromatin-immunoprecipitation-protocol
实验概要The method is a native chromatin immunoprecipitation protocol.主要试剂1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
多功能紫外分析仪使用技巧
多功能紫外分析仪使用技巧一、使用范围介绍:ZF1-II多功能紫外分析仪系,又称、切胶仪、采用不同波长的紫外光对DNA、RNA电泳凝胶样品进行观察拍照、检测蛋白质、核甘酸、适用于核酸电泳分析、检测,PCR产物检测,DNA指纹图谱分析,纸层分析或薄层分析等。紫外分析仪适用于核酸电泳、荧光的分析、检测,
琼脂糖凝胶中DNA的检测
琼脂糖凝胶中的核酸通过染色,可以在波长为 300 nm 的紫外灯下检测。介绍琼脂糖凝胶中核酸染色的两种方法:溴化乙锭(ethidium bromide, EB ) 染色法和 SYBR Gold 染色法。本实验来源「分子克隆实验指南第三版」黄培堂等译。实验方法原理琼脂糖凝胶中的核酸通过染色,可以在波长
DNA酶切及凝胶电泳的相关内容介绍
琼脂糖是从海藻中提取出来的一种多聚多糖,是由D-半乳糖和L-半乳糖以α-1,3和β-1,4糖苷键相连形成的线状高聚物。琼脂糖遇冷水膨胀,溶于热水成溶胶,冷却后成为孔径范围从50nm到大于200nm的凝胶。 琼脂糖或聚丙烯酰胺凝胶电泳是分离鉴定和纯化DNA片段的标准方法。该技术操作简便快速,可以
Genotyping-Transgenic-Rodents-by-PCR
Genotyping Transgenic Rodents by PCRThis is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigators
DNA拓扑异构酶催化反应的相关介绍
很多,其反应本质是先切断DNA的磷酸二脂键,改变DNA的链环数之后再连接之,兼具DNA内切酶和DNA连接酶的功能.然而它们并不能连接事先已经存在的断裂DNA,也就是说,其断裂反应与连接反应是相互耦联的。拓扑异构酶(包括Ⅰ型和II型)都可以用符号转化模型进行解释 除了DNA拓扑异构酶可以产生异构
DNA-laddering-assay-for-treated-cells
Characteristics of this procedure:I found the procedure described by Gong et al. to be a convenient and successful method to detect DNA laddering in c
赛默飞再出手-收购生物过程管理技术企业Finesse-Solutions
分析测试百科网讯 近日,赛默飞宣布,因1976年的 Hart-Scott-Rodino反托拉斯改进法案而对收购Finesse Solutions公司的等待,已收到等待期提前终止的通知。 Finesse Solutions是用于生物生产的可扩展控制自动化系统和软件开发的领先者,位于加利福尼亚州圣
PCR产物纯化方法
Purification of PCR Products in Preparation for CloningJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid
Methylation-Specific-PCR
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
Adaptas-Solutions携多款仪器核心零部件参展BCEIA-2021
2021年9月27日-29日,第十九届北京分析测试学术报告会暨展览会(BCEIA 2021)在北京•中国国际展览中心开幕。在本届展会上,Adaptas Solutions中国公司“江苏艾特斯精密制造有限公司”(以下简称“江苏艾特斯”)的总经理周朋接受了分析测试百科网的采访,为我们带来了展会产品的
叶绿体DNA分离
设备:Hitachi CS-150GXL或CS-120GXL微量超速离心机,S100AT6 转头,5PA 密封管(如果用4PC管,可接比例减少各层液量)溶液配制:A液:0.35Msorbitol(山梨醇),50mM Tris—Hcl (PH8.0) 25mM EDTA—Na2B液:5%(w/w)So
PCR-protocol
PCR reactionProtocol for 50µl reaction - adjust amounts if necessary, for a 20µl reaction use the same volumes of primer and dNTP-mix, but adjust the
哺乳动物RNAi技术-Mammalian-RNA-Interference
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide To Gene S
Experimental-Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Preparation-Of-Ciliated-Protozoa-For-Scanning-Electron-Microscopy
Preparation Of Ciliated Protozoa For Scanning Electron MicroscopyGeneral notes: The same procedures are used to fix and stain cells for SEM and for TE
从棉花组织中提取微量RNA的标准实验方法protocol
Prior to Extractions ・ Bake all necessary glassware, metal spatulas, mortars, and pestles overnight in a 200℃ oven after wrapping them in aluminum f
基于PCR技术的染色质沉淀分析
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a loc
核酸电泳的指示剂与染色剂
指示剂 核酸电泳常用的指示剂有溴酚兰和二甲苯青及银染色.溴酚兰在碱性液体中 呈紫兰色,在0.6%、1%、1.4%和2%琼脂糖凝胶电泳中,溴酚兰的迁移率分别与1Kb、 0.6Kb、0.2Kb和0.15Kb的双链线性DNA片段大致相同.二甲苯青的水溶液呈兰色,它在 1%和1.4%琼脂糖中电泳时,
核酸电泳的指示剂与染色剂
指示剂 核酸电泳常用的指示剂有溴酚兰和二甲苯青及银染色.溴酚兰在碱性液体中 呈紫兰色,在0.6%、1%、1.4%和2%琼脂糖凝胶电泳中,溴酚兰的迁移率分别与1Kb、 0.6Kb、0.2Kb和0.15Kb的双链线性DNA片段大致相同.二甲苯青的水溶液呈兰色,它在 1%和1.4%琼脂糖中电泳
EB染色液使用说明
产品简介:EB染色液是EB的水溶液,浓度为10mg/ml。EB(溴化乙锭)能与核酸分子特异结合,用于观察琼脂糖或聚丙烯酰胺凝胶中DNA或RNA分子。广泛应用于核酸电泳前后的染色以及流式细胞仪的样品染色处理等。溴化乙锭(Ethidium Bromide;C21H20N3Br;分子量为394.32)含有
核酸电泳指示剂和染色剂的选择
指示剂:核酸电泳常用的指示剂有溴酚兰和二甲苯青及银染色.溴酚兰在碱性液体中 呈紫兰色,在0.6%、1%、1.4%和2%琼脂糖凝胶电泳中,溴酚兰的迁移率分别与1Kb、 0.6Kb、0.2Kb和0.15Kb的双链线性DNA段大致相同.二甲苯青的水溶液呈兰色,它在 1%和1.4%琼脂糖中电泳时
Electrophoresis-of-PCR-products-with-Sunrise-gel-apparatus
Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel: In a 500 ml Pyrex® glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
Quick-and-reliable-method-to-analyze-meiotic-segregation-patterns
It is well known that multiple auxotrophic markers impede fruiting in Coprinus cinereus. Restriction fragment length polymorphisms have been used to a
Northern-Blotnorthern杂交
Northern BlotPreparation of Formaldehyde Agarose GelThe gel conditions (1% agarose, 1X MOPS, 6.3% formaldehyde) are designed for ~4 hours of electroph
RNA-analysis-on-nondenaturing-agarose-gel-electrophoresis
实验概要RNA analysis on non-denaturing agarose gel electrophoresis实验步骤1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
RNA-analysis-on-nondenaturing-agarose-gel-electrophoresis
1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer instead of 1X TBE- use agarose gel in the concentration of 1.1%-1.
DAPI染色液的使用方法及注意事项
DAPI染色液(DAPI Staining Solution)是经过精心优化几乎适用于所有常见细胞和组织细胞核染色的染色液。DAPI,即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,也称DAPI dihydrochloride,分子式
MetalEnhanced-Immu...-(二)
实验步骤1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated on slide substrates at 3,000 rp
Methods-for-the-Detection-of-DAminoAcid-Oxidase1
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de