Quickandreliablemethodtoanalyzemeioticsegregationpatterns
It is well known that multiple auxotrophic markers impede fruiting in Coprinus cinereus. Restriction fragment length polymorphisms have been used to advantage (Cassidy et al. 1984 Curr. Genet. 8:607-613; Zolan et al. 1992 Nucl. Acids Res. 20:3993-3999) but the required Southern analysis is both time consuming and laborious. Here we demonstrate a quick and reliable method using the polymerase chain reaction (PCR)......阅读全文
Quick－and－reliable－method－to－analyze－meiotic－segregation－patterns
It is well known that multiple auxotrophic markers impede fruiting in Coprinus cinereus. Restriction fragment length polymorphisms have been used to a
A－quick－method－to－isolate－plant
Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.
Meiotic－Syncrhony－for－SK1
1. Inoculate the entire colony in 5 ml YPD culture O.N. or 12 hours2. Dilute into YPA medium (1% yeast extract, 2% Bacto-peptone, 2%KOAC) to OD600~ 0.
拉曼光谱Analyze－IQ化学软件
化学计量包是拉曼分析的有力助手对于模块化的海洋光学拉曼系统来说，在光谱分析和化学计量方面，Analyze IQ软件是一个有益补充。对于采用我们的光谱仪、 激光器及附件产品的模块化拉曼系统的用户，Analyze IQ化学计量软件包提供了丰富的拉曼光谱库，满足了近1,900种实验室材料、溶剂等
Quick－and－Easy－Isolation－of－Genomic－DNA－from－Yeast
ProcedureTransfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microcentrif
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Method:－Lymphocyte－Transformation
Method: Lymphocyte TransformationMay 30, 1990Rosalie VeilePrinciple:Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocyte
A－quick－RNA－miniprep－for－Neurospora－mycelial－cultures
Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multipl
ImmunoLaser－Capture－Microdissection
A: Development of Immuno-LCMLimitation of MicrodissectionMicrodissection of routinely stained or unstained frozen sections has been used successfully
酵母准备
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (rapid glass bead
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells1
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Fast－and－reliable－miniprep－RNA－extraction－from－Neurospora－crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extrac
VWR收购Reliable－Biopharmaceutical与BioArra两家公司
全球领先的实验室产品与服务供应商VWR近期收购了两家公司，Reliable Biopharmaceutical 与BioArra。 Reliable Biopharmaceutical是一家为生物制药行业提供高质量活性药物成分（API）、高纯度成分（HPIs）（如cGMP辅料）、高级中间体的厂
Quick－Yeast－DNA－Prep:－Isolation－of－Total－DNA－(genomic－and－plasmid)
Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg.Transfer culture to a small 13 x 100 glass tube. Spin down cells 2 min. in
Easy－YAC－Preparation－Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA[This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw
A－Method－for－Structure5
ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely
A－Method－for－Structure4
We also used this mutant (SMdC) as a host to generate two types of lacZ transcriptional reporter fusion strains to assay the promoter activity of QS-c
A－Method－for－Structure3
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the comC de
A－Method－for－Structure2
CD SpectroscopyCD spectra for peptides and reference solutions were recorded at 298 K on a Jasco J-920 CD spectrometer with a 1-mm quartz cuvette. Spe
A－Method－for－Structure1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
DNA转化
DNA转化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab)Very nice protocol for E. Coli transformation inc
Paraformaldehyde
Mary-Ann Campbell's method.This is a quick method of getting paraformaldehyde (PFM) into solution. It is a good alternative to the traditional met
NUF2基因突变与药物因子介绍
该基因编码一种与酵母nuf2高度相似的蛋白质，nuf2是与着丝粒相关的保守蛋白复合物的组成部分。当着丝粒与纺锤体失去连接时，酵母nuf2在减数分裂前期从着丝粒中消失，并在染色体分离中起调节作用。该编码蛋白与有丝分裂hela细胞的着丝粒相关，提示该蛋白是酵母nuf2的功能同源物。已经描述了编码相同蛋白
NUF2基因编码功能及结构描述
该基因编码一种与酵母nuf2高度相似的蛋白质，nuf2是与着丝粒相关的保守蛋白复合物的组成部分。当着丝粒与纺锤体失去连接时，酵母nuf2在减数分裂前期从着丝粒中消失，并在染色体分离中起调节作用。该编码蛋白与有丝分裂hela细胞的着丝粒相关，提示该蛋白是酵母nuf2的功能同源物。已经描述了编码相同蛋白
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Nicotiana－(Nicotiana－tobaccum,－Nicotiana－benthamiana)
Agrobacterium -mediated transformation of Nicotiana species, namely, Nicotiana tobaccum and Nicotiana benthamiana , using leaf disks as the target e
酵母转化
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· Yeast Transformation (Breeden Lab)LiAc method· Large-Scale Y
Method:－Cell－Counts－Using－a－Hemacytometer
Method: Cell Counts Using a HemacytometerJune 1, 1990Rosalie VeilePurpose:The purpose of this procedure is to determine the cell density of the cultur
Basic－Method－for－Indirect－Immunofluorescence－Labeling
Basic Method for Indirect Immunofluorescence LabelingBackgroundThis is the method for indirect immunofluorescence labeling; that is, the antibodies do
Method:－Maintaining－Lymphoblastoid－Cell－Lines
Method: Maintaining Lymphoblastoid Cell LinesJune 10, 1990Rosalie VeilePurpose:To grow lymphoblastoid cells for permanent storage and for DNA extracti