EthidiumBromideDecontamination
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decontamination of solutions > 0.5mg/mlThis method reduces the mutagenic activity by ~3000 fold and is taken from Quillardet and Hofnung, 1988.1) Add sufficient water to make the concentration < 0.5mg/ml.2) Add 1 volume of 0.5M KMnO4 followed by one volume of ......阅读全文
Cell－Counting/－LiveDead－Discrimination
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the numb
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p
AMNIOTIC－FLUID－CULTURES－ON－COVERSLIPS
I. Purpose:Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities. II. Culture Procedure:A. Aseptic techniq
AMNIOTIC－FLUID－CULTURES－ON－COVERSLIPS
实验概要 AMNIOTIC FLUID CULTURES ON COVERSLIPS主要试剂Solutions:Colcemid working solution: 10 mcg/ml Colcemid in Hank's Balanced Salt Solution, sto
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
甲醛洋菜胶体电泳
甲醛洋菜胶体电泳 (formaldehyde-agarose gel electrophoresis)甲醛是一种常用的RNA 变性剂。在进行甲醛洋菜胶体电泳分析时，必须先配制含有甲醛的洋菜胶体，RNA 也必须先以甲醛及formamide 进行变性处理，以确保其二度结构充分被打开。由于甲醛可能是一种致
Isolation－of－genomic－DNA－from－bacteria
Note: This procedure does not work well with Gram + cocci.Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, decant
CORE－SAMPLE－PCR:－A－method－to－rePCR－unique－bands－from－products－of－mixed－s
INTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often contain
Large－Scale－Plasmid－Preps:－Qiagen/Cesium－Method
Most plasmids can be adequately prepped by kits containing DNA binding columns. These columns do not do a great job of separating plasmid DNA from con
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
关于核酸电泳—琼脂糖凝胶电泳的仪器及试剂介绍
琼脂糖凝胶电泳的仪器及试剂： 仪器设备应包括水平凝胶电泳槽及其配套电泳梳、稳压电泳仪、微波炉或普通电炉。同时配备紫外线检测仪和照相系统。 试剂包括琼脂糖、电泳缓冲液、溴化乙锭溶液、凝胶加样缓冲液。 电泳缓冲液常用TBE(1 000ml中含5.4g Tris，2.75g硼酸，2ml 0.5
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
实验常用试剂的制备与储藏（三）
二.电泳缓冲液、染料和凝胶加样液 电泳缓冲液 50×Tris-乙酸（TAE）缓冲液 5×Tris-硼酸（TBE）缓冲液 染料 1％溴酚蓝（bromophenol blue） 加1g水溶性钠型溴酚蓝于100ml水中，搅拌或涡旋混合直到完全溶解。 1％二甲苯青FF（xylene
TISSUE－CULTURE－ON－COVERSLIPS
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
CORE－SAMPLE－PCR
A method to re-PCR unique bands from products of mixed sizeContentsINTRODUCTIONPROTOCOLCOMMENTSINTRODUCTIONThe products of a PCR reaction - especially
甲醛洋菜胶体电泳（formaldehydeagarose－gel－electrophoresis）
甲醛洋菜胶体电泳 （formaldehyde-agarose gel electrophoresis）甲醛是一种常用的RNA 变性剂。在进行甲醛洋菜胶体电泳分析时，必须先配制含有甲醛的洋菜胶体，RNA 也必须先以甲醛及formamide 进行变性处理，以确保其二度结构充分被打开。由于甲醛可能
Easy－YAC－Preparation－Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA[This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS2
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role in many physiological
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
RNA－Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
Agarose－Gels－for－Single－Stranded－DNA
1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the f
吖啶橙的基本信息介绍
简称：AO AO能与无机酸形成盐，具有绿色荧光。如果再稀释的话，便水解成游离的AO，而呈现紫色荧光。 吖啶橙/溴化乙锭双荧光染色测定PC12细胞凋亡（acridine orange/ethidium bromide, AO/EB）： （1）细胞爬片：在6孔培养板中预先置入玻璃盖玻片，接种细
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
重组DNA的分离、克隆与测序实验手册2
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
甲醛洋菜胶体电泳
实验概要本实验介绍了甲醛洋菜胶体电泳 (formaldehyde-agarose gel electrophoresis)的操作流程。实验原理甲醛是一种常用的RNA 变性剂，进行电泳时所使用的缓冲液为MOPS (3-[N-morpholino] propanesulfonic acid)。由于r
Fast－and－reliable－miniprep－RNA－extraction－from－Neurospora－crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extrac
Methylene－Blue－DNA－staining－protocol
Methylene Blue DNA staining protocolProtocol:Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained ge
DNA－Purification－from－Agarose－Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel wit
Engineering－BioBrick－vectors－from－BioBrick－parts/Colony－PCR
MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')De