DNAPurificationfromAgaroseGels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel with a razor blade above and below the bands of interest. Insert the membrane into the gel at each slice, avoiding air bubbles between the gel and the membrane. The membranes above the bands of interest prevent contamination with higher molecular weight DNA.3. Electrophore......阅读全文
DNA－Purification－from－Agarose－Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel wit
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
Agarose－Gels－for－Single－Stranded－DNA
1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the f
Preparation－of－Agarose－Gels－for－DNA－separations
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add 0
DNA－Purification－from－Blood－or－Body－Fluids
实验概要试剂盒，操作简单，稍有点贵，还可以接受。实验材料blood实验步骤Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
EGel®－CloneWell－Agarose－Gels
实验概要Instructions are provided below for using the E-Gel®CloneWell pre-cast agarose gels with the E-Gel® iBase™ Power System. For detailed instructio
Ligation－Optimization
The following protocol can be used to optimize ligation conditions for difficult to clone (e.g. very large) fragments. The principle is to independent
Purification－of－poly(A)+－RNA－fromnb
0. (Optional)Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to remove fine particle of latex. To wash the latex, transfer appropriate a
RNA－Collection－－Purification－from－fibrous－tissue
实验概要Provides an easy and fast method for isolating total RNA from fibrous tissues which contains contractile proteins, connective tissue and colla
Purification－of－acidic－phosphatase－from－mustard－seedlings
Purification of acidic phosphatase from mustard seedlings Phosphate esters are widely distributed in any organism. Nucleic acids, metabolic intermedia
酵母准备
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (rapid glass bead
RNA－Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
PCR产物纯化方法
Purification of PCR Products in Preparation for CloningJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid
DNA－mobility－in－gels
1. Migration of marker dyes in native polyacrylamide non-denaturing gels Gel % Bromophenol blue (BP) Xylene cyanole (XC) 3.5 100 460 5.0
DNA－Sequencing－Gels
DNA Sequencing GelsBuffers and gel solutionsLong Ranger: we started using this in early 1995. Great stuff; the best thing is that the gels are not sti
RNA电泳
RNA Gel (Crawford Lab)Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis.Northern Gel and TransferUsing glyoxal
Sauer:RNA－Purification－from－E.－coli
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
RNA电泳
· RNA Gel (Crawford Lab)· Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis. · Northern
Edman－Sequencing－of－Proteins－from－2D－Gels
The Western blotting/sequencing technique using polyvinylidene difluoride (PVDF) membrane is one of the most popular technique for Edman sequencin
Southen杂交
Southern杂交One important thing for transfer:the weight of the object resting on top of the blotting apparatus should not exceed the weight equal to a 5
Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should
Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants主要试剂 Protein
ELECTROPHORESIS－OF－DNA－IN－POLYACRYLAMIDE－GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Anal
Factor－Xa－Cleavage－of－MBPFusion－protein
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The
寡核苷酸的相关操作
In this section, you will find techniques related to oligonucleotides, such as oligo purification by acrylamide gel, annealing two oligos to make doub
Facts－and－trouble－shooting
10 Fun Facts for DNA Electrophoresis::Migration of DNA is retarded and band distortion can occur when too much buffer covers the gel. The slower migra
重组DNA的分离、克隆与测序实验手册2
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
PCR的下游应用
· Agarose Gel Electrophoresis of PCR Products (Robert H. Cruickshank)· Agarose Gel Electrophoresis of PCR Products (Immunology Resourc