新技术：InCellWesternAssay2
III. Experimental ConsiderationsProper selection of microplates can significantly affect the results of your analysis, as each plate has its own characteristics including well depth, plate autofluorescence, and well-to-well signal crossover. Use the general considerations for microplate selection provided below.In-Cell Western analyses use detection at the well surface with no liquid present. This results in minimal ......阅读全文
新技术：InCell－Western－Assay
In-Cell Western AssayComplete Sample Protocol Detailing the SeedingStimulation, and Detection of the HeLa CellularResponse to Epidermal Growth FactorI
新技术：InCell－Western－Assay2
III. Experimental ConsiderationsProper selection of microplates can significantly affect the results of your analysis, as each plate has its own chara
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part
DGK－Assay
Buffers: - 2X buffer 10 ml 0.5 M imidazol, pH 6.6 0.21 g LiCl 1.25 ml 1 M MgCl2 1.0 ml 0.1 M EGTA, pH 6.6 --> Bring volume up to 50 ml with distille
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Assay－of－Phospholipase－A－Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids a
Actin－Capture－Assay
David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix
Needle－Assay－for－Chemotaxis
Devreotes Lab, John Hopkins Medical Institutions http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm Equipment and chemicals Zeiss
Assay－for－the－Micrococcal－Nuclease
Method: Essentially that described by Heins et al. (1966) based upon the release of acid soluble oligonucleotides following nuclease digestion of DNA
BIURET－PROTEIN－ASSAY
BIURET PROTEIN ASSAY MATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard d
In－vitro－Sphingomyelinase－Assay
Reagents: Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 µg/ml CLAP 1 mM PMSF Buffer A 10 mM MgCl2 0.2 M Tris-HCl, pH 7.4 0.2 % Triton X
ELISA－Inhibition－Assay
ELISA Inhibition AssaySensitize a 96-well microtiter plate with purified antigen.Prepare a solution of the purified antigen of interest in phosphate b
cell－proliferation－assay
cell proliferation assaybefore start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until c
Leaf－GUS－Assay
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-L
HISTONE－KINASE－ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro
Pheromone－Halo－Assay
-Use sterile technique and sterile solutions throughout this method.-1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturat
Glucosamine－Rapid－Assay
Glucosamine Rapid AssayMRTHOD:Place sample (containing 0.5 - 10 µg GlcN) in a Pyrex screw capped tube.Add HCl to a final concentration of 2N and a fin
Leaf－GUS－Assay
实验概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Tube－formation－assay
DescriptionThis is a fast and easy assay to test the angiogenic/anti-angiogenic properties of molecules. As compared to other angiogenesis assays, suc
Bradford－protein－assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly