Tubeformationassay

Tube－formation－assay

DescriptionThis is a fast and easy assay to test the angiogenic/anti-angiogenic properties of molecules. As compared to other angiogenesis assays, suc

SOFT－AGAR－ASSAY－FOR－COLONY－FORMATION

Note: All volumes are calculated to cater for four plates per point.Base Agar1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. War

Single－tube－confirmation－PCR－protocol

The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat

使用HUVEC细胞进行血管生成实验，并用LONZA核转仪转染a...

使用HUVEC细胞进行血管生成实验，并用LONZA核转仪转染anti-VEGFR2 siRNASrinivasan Kokatam1, Kanchan Tiwari1, Jenny Schroeder2, Andrea Toell2, Lubna Hussain3, Preeti Kapoor1.1

Testing－the－effects－of－retinoic－acid－on－tail－formation

The－Effects－of－NiCl2on－Spicule－Formation

The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol

Formation－of－Ketone－Bodies－from－acetylCoA

The acetyl-CoA produced by mitochondrial beta-oxidation of fatty acids enters the Kreb's cycle to produce energy, but that is not the only fate of

Joint－formation－in－chick－limb－bud－CAM－grafts

Abstract        Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive events of limb formation. Experimenters hav

Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates

AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helicobacte

GST－Activity－Fluorometric－Assay

实验概要The  experiment provides a simple, fluorescence-based in vitro assay for  detecting the GST activity using a fluorescence plate reader. The assay

Bradford－Assay

Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B

MTT－Assay

 This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in

Protease－assay

实验概要        In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in

Aspartate－Assay

实验概要The  Aspartate Assay Kit provides a simple, convenient assay to measure  aspartate in a variety of samples. In the assay, aspartate is converted  

Phosphate－Assay

1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry

Protease－assay

In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe

Polygalacturonase－assay

This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o

DGK－Assay

Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.

Motility－Assay

DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o

Bradford－Assay

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue

Chemotaxis－Assay

PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel

Pectinase－assay

Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w

TUNEL－assay

PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie

Cellulase－assays

实验概要        Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the frui

Cellulase－assays

Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p

TUBE1基因的结构特点和作用

这个基因编码微管蛋白超家族的一个成员。该蛋白定位于中心体复制后与两个中心粒中较老的中心粒相关的中心粒近远侧附属物。这种蛋白在中心粒复制过程中的微管组织中起着中心作用。在5号染色体上发现了该基因的假基因。

HISTONE－KINASE－ASSAY

PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro

Cell－Viability－Assay

Dye exclusiona cell suspension is mixed with trypan blue and examined by low-power microscopyMaterialscellsPBSM3hemocytometer0.4 % trypan blue in PBSm

LOWRY－PROTEIN－ASSAY

The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-

ELISA－Inhibition－Assay

ELISA Inhibition AssaySensitize a 96-well microtiter plate with purified antigen.Prepare a solution of the purified antigen of interest in phosphate b