MagneticDepletionofSSEA4+UndifferentiatedEmbryonicStemCells

实验概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for the magnetic depletion of SSEA-4 undifferentiated embryonic stem cells from a differentiated cell population to increase purity. Isolated SSEA-4 negative cells are bead- and antibody free and are suitable for any downstream application. The produ......阅读全文

Mouse-B-cell-isolation-with-magnetic-beads

实验概要Isolate B cells from total mouse cells实验步骤 Praparation:1.       Enough MACS; put RPMI into water bath.2.       Put some MACS in 15 ml tube or 1.5

Magnetic-Depletion-of-SSEA4+-Undifferentiated-Embryonic-Stem-Cells

实验概要Human  embryonic stem cells can be differentiated into neural-, mesenchymal  and hematopoetic stem cells. This product is intended for the magneti

NMR(Nuclear-Magnetic-Resonance)为核磁共振的应用介绍

核磁共振适合于液体、固体。如今的高分辨技术,还将核磁用于了半固体及微量样品的研究。核磁谱图已经从过去的一维谱图(1D)发展到如今的二维(2D)、三维(3D)甚至四维(4D)谱图,陈旧的实验方法被放弃,新的实验方法迅速发展,它们将分子结构和分子间的关系表现得更加清晰。在世界的许多大学、研究机构和企业集

链霉亲和素磁珠(SA-magnetic-bead)性能比较研究

磁性聚合物微球兼具高分子微球特性和磁响应性,因此,广泛应用于靶向给药、生物诊断、催化剂制备、磁成像等领域。目前,大部分的生物分子是通过表面的氨基或者羧基与磁性微球表面的基团共价结合的方式直接固定化在磁性微球的表面。这种方法不仅复杂,还会使固定化的生物分子活性受到较大影响,例如抗体可能会失去与抗原的特

蛋白偶联到磁性MagPlex™微球的方法

Sample Protopcol  for  Two-Step Carbodiimide  Coupling  of  Protein to MagPlex™ Magnetic  Carboxylated MicrospheresMicrospheres should be protected fr

Isolation-Of-PCR-Products

实验概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.实验原理The ChargeSwitch® TechnologyT

High-Throughput-Isolation-Of-PCR-Products-Using-ChargeSwitch®-PCR-CleanUp

实验概要The ChargeSwitch®  PCR Clean-Up Kit allows rapid and efficient purification of PCR  products from salts, primers, dNTPs, and other non-nucleic aci

Automated-Genomic-DNA-Extraction

实验概要This section  provides a general protocol for automated isolation of genomic DNA from  10-20 µl blood samples in a 96-well format using the Charge

DNA-Extraction-from-Blood

实验概要The ChargeSwitch®  gDNA Purification Kits allow rapid and efficient purification of  genomic DNA from small volumes of human blood. After preparin

Automated-Extraction--Normalized-DNA-Buccal-Kit

实验概要This section  provides a general protocol for automated isolation of genomic DNA from  human buccal cell swabs in a 96-well format using the Charg

磁共振的基本信息介绍

  磁共振指的是自旋磁共振(spin magnetic resonance)现象。其意义上较广,包含核磁共振(nuclear magnetic resonance, NMR)、电子顺磁共振(electron paramagnetic resonance, EPR)或称电子自旋共振(electron

Isolation-of-Genomic-DNA-from-Tissue-Using-ChargeSwitch®-Technology

实验概要 The ChargeSwitch®  gDNA Mini and Micro Tissue Kits allow rapid and efficient purification  of genomic DNA from mini (10-25 mg) or micro (3-5 mg)

毫米波隔离器最新技术进展(一)

Recent Advancements in mmWave Isolator TechnologyMarch 13, 2019 David W. Porterfield, Jr., Micro Harmonics Corp., Fincastle, Va. No CommentsIsolators

WBand-FrequencySwept-EPR(二)

The EPR signal from the sample resonator is amplified in a W-band low-noise amplifier (LNA) and downconverted to 35 GHz in the W-band downconver

毫米波隔离器最新技术进展(二)

The traditional method to tune Faraday rotation isolators is to use ferrites substantially longer than the minimum required length and adjust th

WBand-FrequencySwept-EPR(一)

W-Band Frequency-Swept EPR毫米波(W频段 75-110GHz)频率扫描EPR系统实验James S. Hyde,a,* Robert A. Strangeway,a,b Theodore G. Camenisch,a Joseph J. Ratke,a and Wojcie

The-E.Z.N.A.®-MagBind™-Dye-terminator-Removal-Procedure

实验概要Excess  unincorporated, nonradioactive label can cause high background  fluorescence in automated sequencing gels. For optimal sequencing  results

核磁共振成像简介

  核磁共振成像(英语:Nuclear Magnetic Resonance Imaging,简称NMRI),又称自旋成像(英语:spin imaging),也称磁共振成像(Magnetic Resonance Imaging,简称MRI),是利用核磁共振(nuclear magnetic reso

FDA关于粗品肝素钠来源检测的方法(二)

l. 上下吹打5次混匀。m. 室温孵育1min。n. 把离心管放到MagnaRack上直至ChargeSwitch Magnetic Beads形成紧密沉淀,上清变清澈(约1min)。o. 离心管留在磁铁上,小心吸掉上清不搅浑沉淀。吸取上清时枪头不要对着沉淀物。p. 从磁铁取下离心管。q. 加入1m

可靠的毫米波正交干涉仪(70GHz)(二)

Many interferometers are required to operate in high ambient magnetic fields (B-fields), e.g. around magnetic confinement plasma devices. Typicall

核磁共振的原理

NMR(核磁共振)nuclear magnetic resonance。A phenomenon in which transitionsin the magnetic energy states of the nuclei of atoms are induced when the atoms a

核磁共振的原理

NMR(核磁共振)nuclear magnetic resonance。A phenomenon in which transitionsin the magnetic energy states of the nuclei of atoms are induced when the atoms a

WBand-FrequencySwept-EPR(四)

3. Discussion3.1. Frequency-sweep rateProspects for enhancement of peak frequency deviation and frequency sweep rates in a YTO are primarily limit

磁珠法提取核酸的操作技巧

最近有不少小伙伴问到磁力架,着实令我有点意外了,之前一直以为大家还是比较热衷柱式提取法,毕竟小编之前读研的时候,柱提法是我们实验室历代师兄师姐一路传承下来的,我们压根就没想过还有更快更便捷的方法,今天我们就来聊一聊这个已经开始流行的用于核酸(DNA&RNA)的纯化、片段分选的磁珠法。  说到磁珠法,

High-Resolution-Agarose-Gel-Electrophoresis

实验概要Agarose gel  electrophoresis remains the most widely used technique for separating  nucleic acid fragments due to its ease of use, non-toxicity, a

泛素化蛋白快速富集纯化黑科技TUBEs

       泛素化是一种常见的调节蛋白的稳定性和功能的翻译后修饰。蛋白的泛素化需要3种酶的参与,分别是泛素活化酶(ubiquitin-activating enzyme,E1)、泛素偶联酶(ubiquitin conjugation enzyme,E2)和泛素连接酶(ubiquitin

Basic-Theory-and-Use-of-GCMS(二)

For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction,

磁性半导体的定义

磁性半导体(英语:Magnetic semiconductor)是一种同时体现铁磁性(或者类似的效应)和半导体特性的半导体材料。

NDT无损检测中UT和MT是什么意思

NDT 无损检测中UT和MT是什么意思?UT: Ultra-sonic test 超声波探伤MT: Magnetic test 磁粉探伤磁粉探伤还有另外一个名称:MPI,也就是Magnetic Particle Inspection另外,NDT的英文是Non Destructive Testing。

iPrep™-GeneCatcher™-gDNA-Blood-Kit

实验概要The iPrep™  GeneCatcher™ gDNA Blood Kit allows rapid and automated extraction of  genomic DNA (gDNA) from human blood including archived or poorly