CompleteMouseNecropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to the animal and provides biologically meaningful samples. Only personnel with appropriate AHR training may handle live animals.Isoflourane inhalation: This should be given in sufficient dose to cause complete loss of breathing and pulse in the mouse. Apply 1 mL to a g......阅读全文
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
Preparation of Luciferin forIn Vitro Bioluminescent AssaysMaterials• D-Luciferin Firefly, potassium salt, 1.0 g /vial(Caliper Life Sciences Part Numbe
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscience Center
从小鼠和人中克隆miRNA实验方法
Supplementary information to miRNAs cloned from mouse and humanAn online clearinghouse for miRNA gene name assignments (http://www.sanger.ac.uk/Softwa
siRNAs结合生物芯片的实验设计1
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating t
immunofluorescence－of－mouse－antihuman－IL6－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of mouse anti-human IL-6 by Peprotech.实验步骤The following protocol used human endomet
immunohistochemistry－of－mouse－antihuman－Eotaxin2－by－Peprotech
实验概要The following protocol provides a method of immunohistochemistry of mouse anti-human Eotaxin-2 by Peprotech.实验步骤The following protocol used formal
Mouse－Big－Endothelin,Big－ET－ELISA－Kit使用原理
Mouse Big Endothelin,Big ET ELISA Kit使用原理小鼠大内皮素(BigET)ELISA试剂盒货号:FK-QZ3291规格：96T/48TMouse Big Endothelin,Big ET ELISA Kit使用原理The kit was a solid pha
immunohistochemistry－of－mouse－antihuman－sIL2R－by－Peprotech
实验概要The following protocol provides a method of immunohistochemistry of mouse anti-human sIL-2R by Peprotech.实验步骤The following protocol used human fro
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of an eight-we
TCell－Activation－Using－mAb－to－CD3
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Isolation,－Culture,－Characterization－of－Cortical－and－Hippocampal－Neurons
实验概要The ability to culture primary neurons under serum-free conditions facilitates tighter control of neuronal studies. Some serum-free media and s
Culturing－BG01V－Human－Embryonic－Stem－Cells－with－Mouse－Embryonic－Fibroblast
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明
Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒货号:FK-QZ2967规格：96T/48TMouse hepatitis B virus e antigen,HBeAg ELISA Kit
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
Cellular－－Molecular－Pathology－Branch
VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP) (http://ntp.niehs.nih.gov/) interdisciplinary research pr
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
Mouse－Pulmonary－surfatcantassociated－protein－A,SPA－ELISA－Kit使用说明
Mouse Pulmonary surfatcant-associated protein A,SP-A ELISA Kit使用原理小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒货号:FK-QZ3080规格：96T/48TMouse Pulmonary surfatcant-associa
用CRISPR/Cas9对CART细胞进行多重基因编辑（三）
流式细胞术 Flow cytometry CytoFLEX (Beckman Coulter Inc) was used to perform fluorescent expression analysis. Cells were harvested on the following day
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967
小鼠（Mouse）肺炎病毒（PVM）ELISA检测试剂盒使用说明
本试剂盒只能用于科学研究，不得用于医学诊断小鼠（Mouse）肺炎病毒（PVM）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗一步夹心法酶联免疫吸附试验（ELISA）。往预先包被小鼠肺炎病毒（PVM）抗体的包被微孔中，依次加入标本、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TM
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
PriCells:－Questions
Questions 1: Isolation of hepatocytes from other speciesThe basic two-step perfusion procedure can be used for obtaining hepatocytes from various r
小鼠（Mouse）甘露糖结合蛋白/甘露糖结合凝集素（MBP/MB...
小鼠（Mouse）甘露糖结合蛋白/甘露糖结合凝集素（MBP/MBL）ELISA检测试剂盒使用说明本试剂盒只能用于科学研究，不得用于医学诊断小鼠（Mouse）甘露糖结合蛋白/甘露糖结合凝集素（MBP/MBL）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）
小鼠（Mouse）乙酰胆碱（ACH）ELISA检测试剂盒使用说明
小鼠（Mouse）乙酰胆碱（ACH）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被乙酰胆碱（ACH）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并
viral－immunity－－pathogenesis－group
OUTLINEWe consider manual counts as less quick but more precize than authomatic (electronic) since in the latter case the counts are based on the size
Protocol－for－Dual－Pulse－Labeling－Using－EdU－and－BrdU－Incorporation
实验概要The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation is traditi
Dual－and－TripleCo...－（二）
主要试剂1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me