CompleteMouseNecropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to the animal and provides biologically meaningful samples. Only personnel with appropriate AHR training may handle live animals.Isoflourane inhalation: This should be given in sufficient dose to cause complete loss of breathing and pulse in the mouse. Apply 1 mL to a g......阅读全文
Complete－Mouse－Necropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
Complete－PCR－Guide
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Mouse－Spleenectomy
OUTLINEPROTOCOLGeneral anesthesia1. inject i.p. 250µl/mouse of the ANESTHETIC mixture < wait for 2-10min for the anesthetic effect>2. for verify the a
Preparation－of－Mouse－Neutrophils
实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline solution [HBSS; Invitrogen, Grand Isla
Pathology－and－Autopsy－of－a－mouse
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixation
Preparation－of－Mouse－Neutrophils
实验概要Preparation of Mouse Neutrophils实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline so
Mouse－keratinocyte－cultures
PRIMARY MOUSE KERATINOCYTE CULTURESIsolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
RNAse－A－Treatment－of－Mouse－Cells
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
Mouse－Compete－Blood－Counts
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4Cl, 29.7 mM NaHCO3, 25 µM Na2EDTA)20.75g. NH4CL2.
Chick－or－Mouse－embr...
实验概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
RAT/MOUSE－GROWTH－HO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Th17－Polarization－of－Mouse－CD4－Cells
实验概要T helper 17 (Th17) cells are a subset of CD4 T helper cells characterized by their production of IL-17, particularly IL-17A and IL-17F. They are
Th9－Polarization－of－Mouse－Splenocytes
实验概要Th9 cells are a subpopulation of T-helper cells, characterized to be involved in allergic diseases and resistance against intestinal nematodes.
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)
Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouthGavage - - into the stomach via a feedin
Mouse－B－cell－isolation－with－magnetic－beads
实验概要Isolate B cells from total mouse cells实验步骤 Praparation:1. Enough MACS; put RPMI into water bath.2. Put some MACS in 15 ml tube or 1.5
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into an adult mo
Mouse－Desmin,Des－ELISA－Kit使用原理
Mouse Desmin,Des ELISA Kit使用原理适应物种： Human,Rat,Mouse,Rabbit应用： 科研实验检测方法： 酶联检测检测限： 科研数量： 100规格： 96T/48TMouse Desmin,Des ELISA Kit使用原理小
Mouse－Eotaxin－2－ELISA－Kit使用原理
Mouse Eotaxin 2 ELISA Kit使用原理小鼠嗜酸粒细胞趋化蛋白Eotaxin2(Eotaxin2/CCL24)ELISA试剂盒货号:FK-QZ3338规格：96T/48TMouse Eotaxin 2 ELISA Kit使用原理The kit was a solid phase
Eccles:Protein－Lysates－from－Tissue
Cell Lysis Buffer5mL 0.1M Tris HCl pH 8 (10mM)0.44g NaCl (150mM)0.02g EDTA (1mM)0.5mL nonidet P40 (1% w/v)0.05g SDS (0.1% w/v)Make up to 50mL with MQH
Mouse－p27－PCR－Using－Gitschier－Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGTGCCATCTCTAT) produce a 0.5kB PCR product.Wildtype allele: mgK-3 (above
Mouse－Collagenase－I－ELISA－Kit使用说明
Mouse Collagenase I ELISA Kit使用说明书Mouse Collagenase I ELISA Kit使用原理小鼠胶原酶I(CollagenaseI)ELISA试剂盒货号:FK-QZ2987规格：96T/48TMouse Collagenase I ELISA Kit使用
Mouse－Renin－ELISA－Kit英文使用说明
Mouse Renin ELISA Kit使用原理 小鼠肾素(Renin)ELISA试剂盒货号:FK-QZ3075规格：96T/48TMouse Renin ELISA Kit使用原理The kit was a solid phase sandwichenzyme-linked immunoso
E.Z.N.A.®－Protocol－for－Mouse－Tails－Snips
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to