GuidelinesforRetroorbitalBleedinginLaboratoryRatsandMice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samplesRack to hold tubesFine tip marker to label tubesAnesthetic agent as outlined in your approved protocolAnesthesia jar with screening materialPaper towels(3cc syringe) optional(20g needle) optionalProcedure:1.Anesthetize mouse or rat (e.g., with metofane, ketamine/xyla......阅读全文
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal6
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal7
ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal3
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other AnalgesicsAnalgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal an
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into an adult mo
Bleeding－Time－Test
OUTLINEAlthough in the Review Article : "Idiopathic Thrombocytopenic Purpura: A practice Guideline Developed by Explicit Methods for the American Soci
5种重要的采血姿势
尾尖采血麻醉小鼠后，用温水擦拭尾巴，引起轻微的血管扩张。但水温不要过高。用无菌手术刀、刀片或锋利的剪刀，快速截断小鼠尾尖0.5-1cm。如果需要多次采血，之后每次仅需截除2-3mm。可以从尾部向尾尖方向按摩，以增加血流。但是，这会降低血样的质量，增加溶血的风险。可以用毛细采血管收集血液，或直接滴入收
Bleeding－and－intravenous－techniques－in－pigs
The ear veinsThe marginal ear veins are the only veins that are easily visible on pigs of any size. Usually there are three prominent veins. The later
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Bleeding－and－intravenous－techniques－in－pigs2
Bleeding techniques for smaller pigsThis picture depicts the venous drainage in the neck of piglets.A: the cephalic vein. This drains into:B: the exte
Tamoxifen－administration－to－mice
One of the uses of tamoxifen in the lab is the activation of enzymes deactivated via an estrogen-binding domain, like Cre-ERT (Cre-LBD). Tamoxifen is
mice－islet－isolation
1. Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2. Which involves cannulation of the common bile duct and
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
医学研究：电刺激可帮助控制大鼠出血（三）
Finite element computational modeling of the resistive heating during the maximum exposure (150 V, 100 μs/phase, 10 Hz, 30 s) demonstrated that the
Osteoblast－Harvest(Adult－Mice)
MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1 Straight ScissorEtoh Spray bottle1 Bone Scraper1 bottle of PBS2 Forceps (1 medium, 1
LongPCR－Reagents－and－Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Clot－Retraction－Test
OUTLINEAbnormal Clot Retraction indicates on abnormal platelet numbers, abnormal glycoprotein amount or structure, abnormal platelet signaling, abnorm
Dorsal－Root－Ganglion－Neuron－Culture－from－Adult－Rats
DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen co
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam Capri & Daniela BarbieriTHE NUCLEU