MurineSkinTransplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in accordance with institution's SOP)Wet hair back from base of ear with alcoholHarvest donor skin by cutting ear off at basePlace tissue in plate on iceSplit ear: pinch ventral and dorsal sides of tissue at base of ear with rat-tooth forceps; gently pull apart tissue; ......阅读全文
Murine－Skin－Transplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in a
Isolation－of－murine－splenocytes
OverviewIn order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cell
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in huma
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Isolating－Xenograft－Tumor－Cells－for－Tissue－Culture－or－Transplantation
Isolation of T4 cells from xenograft tumors Put mouse downWash skin by dipping mice into ETOHRemove tumor and put into 5ml PBS containing Fungizone an
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.实验步骤The following protocol used B6 mice
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech.实验步骤The following protocol used Trichur
immunofluorescence－of－rabbit－antimurine－VEGF－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.实验步骤The following protocol used normal muri
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.实验步骤The following protocol used B6 mice
Transplantation－of－theeyeforming－region－of－Amphibian－neurula－into－theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Micro
Transplantation－of－Pancreatic－Islets－Intothe－Kidney－Capsule－ofDiabetic－Mice
Preparation of Islets for Transplant (Tx)Under an inverted microscope, hand-pick islets using a P200 pipetman and straight pipet tip from the cultured
Analysis－of－murine－BM:－specimen－choice,－sampling－and－processing.
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in huma
新研究激起IPSCs容易导致免疫反应的争论
日本的研究人员将来自小鼠的诱导多能干细胞(iPSCs) 注射回与该小鼠基因完全相同的小鼠中，并指出这样做并不会引发免疫反应，这一结果与之前另一项研究的结果相矛盾。在那篇文章中，研究人员指出利用该技术会导致机体的免疫反应，进而破坏注入的细胞。这项新研究发表在Nature杂志上，研究者们将iPSCs
CCR3－signaling－in－Eosinophils
Eosinophils are a key class of leukocytes involved in inflammatory responses, including allergic reactions in skin and airway. The eosinophil response
化妆品界的“可口可乐”，skin79成长记！
可口可乐可谓是世界上最伟大的品牌！从1886年生产第一瓶可乐开始，走过一百三十多年，已然超脱了一个单纯品牌的范畴，成为一个符号、一种信仰、一种文化。 《全球最佳品牌》（Best Global Brands）年报曾这样评价可口可乐：有时，一家公司会改变我们的生活，它所凭借的不仅仅是它的产品，还有
GentleMACS/单细胞悬液制备文献集锦
种属 处理组织 文章名称 human
百灵威推荐享誉全球的细胞因子产品，国内现货
细胞因子(cytokine,CK)是一类能在细胞间传递信息、具有免疫调节和效应功能的蛋白质或小分子多肽。在nmol/L或pmol/L水平即显示生物学作用，可广泛调控机体免疫应答和造血功能，并参与炎症损伤等病理过程。 细胞因子研究具有非常广阔的应用前景，它有助于阐明分子水平的免疫调节机理;有
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
Complete－Mouse－Necropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbus, the v
高通量测序技术在肠道菌群多样性研究中的应用（三）
一、饮食、能量摄取、肥胖与肠道菌群之间的关系：1. A core gut microbiome in obese and lean twins——2009《Nature》——if 36.102. An obesity-associated gut microbiome with increase
粪菌移植的概念
粪菌移植”（Fecal microbiota transplantation，FMT），其定义是，将健康人粪便中的功能菌群，移植到患者胃肠道内，重建具有正常功能的肠道菌群，实现肠道及肠道外疾病的治疗。在国际合作组对此词进行统一之前，也被少数人称为Fecal transplantation、Fec
Medical－Aesthetics:－Bespoke－Technology－－Trends－to－Embrace－Natural－looks
The hype bolstered by social media influencers and growing awareness about youthful appearance has surged the demand for medical aesthetic procedures
12篇Lancet/Nature文章被撤回，这项“颠覆性”的技术，为何成为杀人工具？
2011年，使用人工合成POSS-PCU纳米复合结构体植入自体干细胞，进行了首例组织工程气管-支气管移植物的人体成功移植。 2012年8月16日，Paolo Macchiarini团队在Biomaterials（IF=14）在线发表题为“Viability and proliferation
单细胞－RNA－测序揭示了体内不同的细胞异质性
植入体内的生物材料和设备具有广泛的临床应用，如组织再生和细胞移植。在细胞微环境的组成部分中，结构特征（宏观，微观和纳米特征）在指导细胞行为方面起着至关重要的作用。 2021年5月26日，四川大学满毅团队在Science Advances 在线发表题为“Dissecting the microe
同系移植的定义
中文名称同系移植英文名称isogenic transplantation;syngenic transplantation定 义遗传背景完全相同的个体间进行的细胞、组织或器官移植。如同卵双生子或同类系动物不同个体间的移植，移植后不发生排斥反应。应用学科免疫学（一级学科），免疫病理、临床免疫（二级学
Isolation－of－dermal－mast－cell－(DMC)
1. Human DMC were isolated from skin by enzymatic digestion.2. Dermal tissue was obtained from patients with skin after informed consent was given.3.
泰国成功采用自体角膜缘上皮移植手术帮助患者重见光明
泰国玛希敦大学诗里叻医院近日表示，该院首次成功采用角膜缘上皮移植技术，使一位患角膜缘缺陷长达20年的患者重见光明。 目前通过角膜缘上皮细胞移植治疗角膜缘缺陷主要有如下疗法：一是自体角膜缘干细胞体外培养移植法（Cultivated limbal epithelial transplantatio