TransplantationoftheeyeformingregionofAmphibianneurulaintotheflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Microsurgery Preparation1)Place two embryos in the 60 mm petri dish containing Operating solution with antibiotics.2)Using tweezers and forceps dejelly the embryo and remove the membrane around it.3)Prepare 2% agarose-coated operating dishes in 1X HBSt (+/- powdered cha......阅读全文
Transplantation-of-theeyeforming-region-of-Amphibian-neurula-into-theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Micro
Fate-Mapping-Axolotl-Embryos-in-Early-Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Developmental-and-phenotypic-implications-of-transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Axolotl-Protocol:-Transplantation-of-the-Gill-Organ-Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Murine-Skin-Transplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in a
Developmental-Effects-of-Transplantation-of-Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Dorsal-Lip-Transplant-in-Amphibian-Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Yale-University-Policy-for-Aseptic-Amphibian-Survival-Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
Regulative-Development-in-Axolotl-Embryos;-Splitting-the-Heart-Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Isolating-Xenograft-Tumor-Cells-for-Tissue-Culture-or-Transplantation
Isolation of T4 cells from xenograft tumors Put mouse downWash skin by dipping mice into ETOHRemove tumor and put into 5ml PBS containing Fungizone an
Development-of-the-Heart-Morphogenetic-Field-in-the-Axolotl-Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Transplantation-of-Pancreatic-Islets-Intothe-Kidney-Capsule-ofDiabetic-Mice
Preparation of Islets for Transplant (Tx)Under an inverted microscope, hand-pick islets using a P200 pipetman and straight pipet tip from the cultured
与肾癌相关的HLAC基因编码功能描述
HLA-C属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAC基因突变与药物因子介绍
HLA-C属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAC基因编码功能及结构描述
HLA-C属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAC基因编码功能及结构描述
HLA-C属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAB基因编码功能及结构描述
HLA-B属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAA基因突变与药物因子介绍
HLA-A属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAB基因突变与药物因子介绍
HLA-B属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAA基因编码功能及结构描述
HLA-A属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAA基因编码功能及结构描述
HLA-A属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
HLAB基因编码功能及结构描述
HLA-B属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
与结直肠癌相关的HLAC基因编码功能描述
HLA-C属于HLA I类重链相似度。这一类分子是由重链和轻链(β-2微球蛋白)组成的异二聚体。重链锚定在膜上。I类分子通过呈现内质网腔中的肽在免疫系统中起着中心作用。几乎在所有细胞中都有表达。重链约45kDa,其基因包含8个外显子。外显子1编码先导肽,外显子2和3编码α1和α2结构域,两者结合肽,
神经胚的介绍
神经胚(neurula)亦称髓胚。主要是指脊索动物的发生中在原肠形成(或者原条期)之后,从出现中枢神经系统原基的神经板时期开始,到袖经板闭合变成神经管期间的胚胎而言。在这一时期外胚层分化为神经板及表皮区,在神经板之下的为头肠、前索板、脊索原基、预定体节的位置,在多数情况下,表皮区的外胚层被侧板的
粪菌移植的概念
粪菌移植”(Fecal microbiota transplantation,FMT),其定义是,将健康人粪便中的功能菌群,移植到患者胃肠道内,重建具有正常功能的肠道菌群,实现肠道及肠道外疾病的治疗。在国际合作组对此词进行统一之前,也被少数人称为Fecal transplantation、Fec
12篇Lancet/Nature文章被撤回,这项“颠覆性”的技术,为何成为杀人工具?
2011年,使用人工合成POSS-PCU纳米复合结构体植入自体干细胞,进行了首例组织工程气管-支气管移植物的人体成功移植。 2012年8月16日,Paolo Macchiarini团队在Biomaterials(IF=14)在线发表题为“Viability and proliferation
活体成像皮下成瘤实验操作方法
Materials:MDA-MB-231-Luc and HCT116-Luc cellsMice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;4 naïve female BALB/c nude mice;Art
活体成像小鼠皮下瘤模型实验步骤
Luciferin Preparation1. Prepare a stock solution of luciferin at 15mg/ml in DPBS. Filter sterilize through a 0.2 um filter.2. Prepare enough to
同系移植的定义
中文名称同系移植英文名称isogenic transplantation;syngenic transplantation定 义遗传背景完全相同的个体间进行的细胞、组织或器官移植。如同卵双生子或同类系动物不同个体间的移植,移植后不发生排斥反应。应用学科免疫学(一级学科),免疫病理、临床免疫(二级学
SingleCell-Electroporation-in-Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering