ProteinStainingProcedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.Coomassie Blue StainingTIP: All washes and incubations are done with constant, gentle shaking.Immerse gel in 50% ethanol/10% acetic acid for at least 1 hr.Soak in 5% ethanol/5% acetic ......阅读全文
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
蛋白质电泳
蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·
细菌检测
Gram Staining (+\-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description for Gram staining Aci
Western－杂交
Western 杂交（主要内容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and OthersPrepa
Differential－cDNA－Screening－Procedures
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500µg/ml (50µl stock + 950µl PBS).7-Amino-actinomycin (Sigma A-94
Conjugation－of－monoclonal－antibodies
Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety, complete
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
Staining－Methods－for－cell
death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidiu
Alkaline－phosphatase－staining
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should
Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants主要试剂 Protein
Zebrafish－whole－mou...
实验概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
Basic－procedures－for－bacteria－culture2
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
Basic－procedures－for－bacteria－culture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
流式细胞仪技术专辑
最方便的实验干货查询工具微信扫码进入「丁香实验」小程序编辑： 呜咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
High－resolution－negative－staining
High resolution negative staining(From Valentine et al, 1968. Biochemistry 7:2143-52)Rationale: For the highest resolution with negative staining, the
Actin－StainingActin－Staining－Protocol
实验概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins, isolated
Direct/Indirect－Staining－Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
Staining－Methods－for－cell－death
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
Intracellular－Cytokine－Staining－Protocol
实验概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surf
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
流式细胞仪技术专辑
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
Protein－Electrophoresis
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh