MeasurementofCarbonFixationRatesinLeafSamples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a range of light intensities long enough for them to fix significant amounts of carbon. It is necessary to expose identical surface areas under favorable conditions which are identical for all leaves except for light intensity (the experimental variable). A means of ......阅读全文
Measurement－of－Carbon－Fixation－Rates－in－Leaf－Samples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Leaf－GUS－Assay
实验概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Leaf－GUS－Assay
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-L
Fixation－of－Embryos
MEMFA Fix10xMEMFA Salts1 part 10x MEMFA salts1 M MOPS1 part 37% formaldehyde20mM EGTA8 parts water10mM MgSO410x salts can be autoclaved and stored. Tu
悬挂－Hanging－Samples
悬挂 Hanging Samples大组织透明化样品可以通过悬挂的方式放置在样品载物台上，样品最大尺寸是1cm*1cm*1cm。适合样品：水溶性试剂透明化的小鼠胚胎、脑、肺、肝脏、肾脏、胰腺、卵巢等。上样方式：平台配有不同形式的样品粘托和样品托盘，可以将样品通过粘贴悬挂或者托挂的方式放置在载物台上，
包埋－Embedded－Samples
包埋 Embedded Samples可以将部分样品包埋在1-4%浓度的低熔点琼脂糖中，不同浓度的琼脂糖其稳定性不同，对活体样品的影响也不同。适合样品：斑马鱼、果蝇、线虫、拟南介等荧光标记的模式生物。包埋尺寸：平台配有不同规格的毛细管和1ml注射器，样品大小0.23mm-3.06mm，介于毛细管和注
Methanol－Fixation－for－Immunofluorescence
Methanol fixation works by precipitating proteins, and as such it is a quick method (2-5)minutes is enough time for most antibodies/proteins). Diffuse
Estimation－of－Fat－in－Milk－Samples
IntroductionMilk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is nece
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
Estimation－of－Fat－in－Milk－Samples
实验概要Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessar
围封－Enclosed－Samples
围封 Enclosed Samples由于高浓度的琼脂糖浓度会影响部分活体样本的发育和状态，琼脂糖浓度太低又会影响样品的稳定性，此时，我们可以选择将样品围封在FEP管中。适合样品：折射率和FEP、琼脂糖相似的样品，如斑马鱼、果蝇、线虫、拟南介等。样品大小：平台配有不同规格的FEP管，具体围封方式类
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
Generic－Fixation－for－Electron－Microscopy
Generic Fixation for Electron MicroscopyThe best way to fix a sample for electron microscopy is to follow a procedure developed and proven by others.
光合碳循环－（photosynthetic－carbon－cycle）
光合作用中碳同化（二氧化碳转化为糖或其磷酸酯）的基本途径。又称卡尔文循环、还原戊糖磷酸循环、还原戊糖磷酸途径。在绿色植物、蓝藻和多种光合细菌中普遍存在。其他碳同化途径如 C4 途径和 CAM途径（见景天科酸代谢）所固定的 CO2 ，最终仍须通过光合碳循环才能被还原成糖。因此它是地球上绝大部分有机物
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Protein－concentration－of－Laemmli－gel－samples
Protein concentration of Laemmli gel samplesTo 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin
Fixation－of－Cells－Cultured－in－Transwell－Dishes
Fixation of Cells Cultured in Transwell DishesTranswell culture dishes are commonly used to culture cells so that the top and bottom of the cells can
固氮作用（nitrogen－fixation）
分子态氮被还原成氨和其他含氮化合物的过程。自然界氮（N2 ）的固定有两种方式：一种是非生物固氮，即通过闪电、高温放电等固氮，这样形成的氮化物很少；二是生物固氮，即分子态氮在生物体内还原为氨的过程。大气中90％以上的分子态氮都是通过固氮微生物的作用被还原为氨的。生物固氮是固氮微生物的一种特殊的生理功
Tetrahymena－Fixation－for－Transmission－Electron－Microscopy
Tetrahymena Fixation for Transmission Electron MicroscopyPellet Tetrahymena cells in a clinical centrifuge.OPTIONAL: Suspend cells in HNMK (50 mM HEPE
Fixation－and－Embedding－of－Microtubules－for－Electron－Microscopy
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning)Primary fix:2% glutaraldehyde
Chlamydomonas－Fixation－for－Transmission－Electron－Microscopy
Chlamydomonas Fixation for Transmission Electron MicroscopySolutions:Chlamydomonas culture medium + 2% glutaraldehyde (5 ml medium + 0.9 ml 25% glutar
胚胎和成年斑马鱼眼情的组织学准备
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
基于epMotion－5075t系统与KPPA－HyperPlus试剂盒的全自动测序...2
Results and DiscussionThe post-ligation qPCR results were used to calculate the percentage of starting material that was successfully adapter ligate
The－Effect－of－Caffeine－on－the－Heart－Rates－of－ThreeDay－Old－Chick－Embryos
Objective The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treati
SBA5型CO2气体分析仪参与作物对大气变化响应的相关研究2
2011CJ Bernacchi, ADB Leakey, BA Kimball, DR Ort (2011) Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotransp
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
植物叶蛋白（the－plant－leaf－protein）的提取
一、实验目的熟悉植物叶蛋白的几种提取原理和方法，了解其意义及其应用价值。二、实验原理植物叶蛋白或称绿色蛋白浓缩物 (leaf protein concentration，简称LPC)，是从新鲜植物叶片中提取的高质量浓缩蛋白质，不仅是畜禽生长发育和生产畜产品的主要营养物质，而且目前也正成为人类的保
Immunofluorescence－...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec