Invivoevaluationofdrugleadcandidatesbyintravenouscontinuousinfusion
In vivo evaluation of drug lead candidates by intravenous continuous infusionSang Ho LeeWesley ShoopBruce MichaelThomas FelcettoSheo SinghJun Wang , jun_wang2@merck.com, Merck Research LaboratoriesRelated Journal & Article InformationManuscript: 2006-01-00990C Journal: Nature Article Title: Platensimycin is a selective FabF inhibitor with potent antibiotic properties IntroductionNumerous&......阅读全文
Invivo－evaluation－ofdrug－lead－candidates－by－intravenous－continuous－infusion
In vivo evaluation of drug lead candidates by intravenous continuous infusionSang Ho LeeWesley ShoopBruce MichaelThomas FelcettoSheo SinghJun Wang , j
使用复合模式SPE技术去除血浆样品中的聚乙二醇400（三）
将PEG 400从血浆中去除的主要目的就是为了清除可能引发的离子抑制效应。图5为由下面方程式得出的基质效应数据一览，据最新AAPS论文报道6。基质效应 =（含PEG的峰响应值/不含PEG的峰响应值） 图表A所示数据来源于经Oasis MCX萃取去除PEG的血浆样品。图表B所示数据来源于经萃取后又
实验室自动化与筛选协会2013亚洲会展短期培训课程简介
短期培训课程I ：生物制剂研发中的高通量筛选 Junma Zhou，博士，上海药明康德新药开发有限公司 刘洁颖博士，上海药明康德新药开发有限公司 In Scope of Short Course - Overview of high-throughput techniques in
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
FOSB－gene－expression－and－drug－abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
MultiDrug－Resistance－Factors
Cancer cells resistant to a diverse set of hydrophobic drugs are known as MDR cells (multidrug-resistant). Large membrane proteins overproduced in MDR
Bleeding－and－intravenous－techniques－in－pigs
The ear veinsThe marginal ear veins are the only veins that are easily visible on pigs of any size. Usually there are three prominent veins. The later
Apricot－Designs－PP5+1应用实验案例（三）
Predicted Hepatic Clearance ml/min/kg (N=6) aFreshd 56.1% 56.6% Drug Enzymes Responsible forMetabolism Observed in vivo ClSYS ml/min/kg 34.7%Single Do
In－Vivo－Ubiquitination－Assay－by－Agroinfiltration
The ubiquitination/proteasome system is involved in nearly all plant signaling processes. Many signaling components are degraded by the 26S protea
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
Measuring－PLD－Activity－In－Vivo
Phospholipase D (PLD) hydrolyzes structural phospholipids like phosphatidylcholine (PC) and phosphatidylethanolamine (PE) into phosphatidic acid (
Bleeding－and－intravenous－techniques－in－pigs2
Bleeding techniques for smaller pigsThis picture depicts the venous drainage in the neck of piglets.A: the cephalic vein. This drains into:B: the exte
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
实验概要BackgroundThe pharmaceutical and biotechnology industry’s goal is to discover therapeutic agents that are both safe and effective at treating or
In－Vivo－Imaging－of－Far2
In vivo bio-imaging Mice were anesthetized and placed in a custom-made bed, which allowed stable and reproducible imaging of the legs. In vivo scann
In－Vivo－Imaging－of－Far1
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
In－Vivo－Imaging－of－Far3
To determine the minimum dose of Katushka plasmid needed to give detectable fluorescent intensity, we decreased the amount of pTurboFP635 to 0.5 and 1
D荧光素钾盐/钠盐体内使用手册（四）
表3：大鼠和小鼠使用不同的镇静剂药物（IP=intraperitoneal; SC=subcutaneous; PO=per os(by mouth); IV=intravenous）药物大鼠剂量（mg/kg）小鼠剂量（mg/kg）注解Tranquilizers/sedativesAcepromaz
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
Preparation of Luciferin forIn Vitro Bioluminescent AssaysMaterials• D-Luciferin Firefly, potassium salt, 1.0 g /vial(Caliper Life Sciences Part Numbe
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1. Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2. Cells were plated on culture dishes
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
药监局：首批45家重点实验室和依托单位的英文名称确定
2019年8月26日，国家药品监督管理局网站发布《国家药监局综合司关于印发首批重点实验室英文名称的通知》。通知称，根据《国家药监局关于认定首批重点实验室的通知》（国药监科外函〔2019〕82号）认定的实验室中文名称，确定了首批45家重点实验室和依托单位的英文名称。 国家药监局首批重点实验室中英
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
腺相关病毒（AAV）在动物实验中的应用（二）
三、肝脏AAV2、AAV5、AAV7 和 AAV8，其中 AAV7 和 AAV8 的效率是 AAV2 的 10-100 倍。注射部位常选择门静脉、外周静脉和尾静脉。注射病毒滴度一般选择 8×10^10-2×10^12(GC/kg)（人、灵长类）；1×10^11 GC/只鼠，稀释成 50-10
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs). MSCs produce adventitial cells in the human bone marrow microenvir
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－5
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of target in
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－3
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio