GuidelinesfortheUseofAnalgesicsandTranquilizersinLaboratoryAnimal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia or may be administered individually for restraint, sedation or analgesia. Dosages for specific animals are linked from RAR's formulary. Often injectable drugs are used in combinations. These drugs tend to have synergistic effects. Mixing them can significantly r......阅读全文
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other AnalgesicsAnalgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal an
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal3
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal7
ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal6
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
实验概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104) 主要试剂SDS based extraction solution实验步骤1. Harvest cells.1) Try
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
D荧光素钾盐/钠盐体内使用手册（四）
表3：大鼠和小鼠使用不同的镇静剂药物（IP=intraperitoneal; SC=subcutaneous; PO=per os(by mouth); IV=intravenous）药物大鼠剂量（mg/kg）小鼠剂量（mg/kg）注解Tranquilizers/sedativesAcepromaz
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Magnetic－Depletion－of－SSEA4+－Undifferentiated－Embryonic－Stem－Cells
实验概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for the magneti
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Human－DC－Enrichment－Kit
实验概要Enrich untouched Dendritic Cells (DCs) by depleting T cells, B cells, monocytes/ macrophages, NK cells, erythrocytes and most granulocytes from
High－Throughput－Isolation－Of－PCR－Products－Using－ChargeSwitch®－PCR－CleanUp
实验概要The ChargeSwitch® PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic aci
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Sea－Urchin－Animal－Maintenance
The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your
USE－诱变实验
实验材料 带 mutS 表型（例如 BMH71-18) 的转化用大肠杆菌感受态 带 mut+表型的转化用大肠杆菌感受态 试剂、试剂盒
USE－诱变实验
本方法中，两条寡核苷酸引物杂交到变性重组质粒 DNA 双链的同一条链上。一条引物（诱变引物）携带一个拟引进靶 DNA 序列的突变，第二条引物携带一个能破坏质粒单一限制酶位点的突变。本实验来源于分子克隆实验指南（第三版）下册，作者：〔美〕J. 萨姆布鲁克 D.W. 拉塞尔。实验材料带 mutS 表型（
USE－诱变实验
实验材料 带 mutS 表型（例如BMH71-18) 的转化用大肠杆菌感受态带 mut+表型的转化用大肠杆菌感受态试剂、试剂盒 退火缓冲液贮存液合成缓冲液噬菌体 T4DNA 连接酶噬菌体 T4DNA 聚合酶或测序酶单一位点的限制性内切核酸酶琼脂糖凝胶诱变引物选择引物质粒 DNA仪器、耗材 70°C
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known