Paraformaldehyde
Mary-Ann Campbell's method.This is a quick method of getting paraformaldehyde (PFM) into solution. It is a good alternative to the traditional methods which can take hours, and has the advantage that it is so quick that you are more inclined to prepare fresh PFM each time.CAUTION! Flammable. Irritant vapor. Probably carcinogenic.Method0.1 g paraformaldehyde powder in small glass tubeAdd 0.5 ml distilled wate......阅读全文
Paraformaldehyde
Mary-Ann Campbell's method.This is a quick method of getting paraformaldehyde (PFM) into solution. It is a good alternative to the traditional met
ICBR－Flow－Cytometry－Core－Laboratory－Paraformaldehyde－Fixation－of－Cells
BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scat
PREPARATION－OF－2％－FORMALDEHYDE－STOCK－SOLUTION－(2－METHODS)
METHOD 1:Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).Prepare as follows:Add 2 g paraformaldeh
2％多聚甲醛固定剂配方二则
METHOD 1:Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).Prepare as follows:Add 2 g paraformaldeh
GFP骨的冰冻切片实验方法
Protocol for Frozen section of GFP Bone:Fix the bone in 4% Paraformaldehyde at 4�C under constant agitation for 3 days.Decalcification in 14% EDTA sol
Formaldehyde－Treatment－of－Tissue－Culture－Hoods
You will need:-12g Potassium Permanganate 6g Crushed paraformaldehyde1) Set the hood so that it can vent outside.2) Mix the two chemicals above togeth
甲醛处理细胞培养中的物品
You will need:-12g Potassium Permanganate 6g Crushed paraformaldehyde1) Set the hood so that it can vent outside.2) Mix the two chemicals above togeth
标本必须在抗体染色后30分内检测吗？
SOP是这么说的：（1）Keep samples after staining covered with aluminum foil and at 4 0C （in the refrigerator） until they are run for flow analysis. The sampl
IHC－frozen－sections...
实验概要The method provides a guideline procedure and tips for staining of frozen sections.实验步骤Frozen sections: Once mounted on APES coated slides, frozen
Measurement－of－GFP－Expression－and－DNA－Content－in－Permeabilized－Cells
ReagentsCells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control.1 X PBS2% Buff
In－Cell－ELISA
实验概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are availabl
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL检测凋亡
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
Staining－Methods－for－cell－death－Z.－Xia－10/2/95
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
Staining－Methods－for－cell－death
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
Staining－Methods－for－cell
death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidiu
组织学——试剂和溶液
Fixative Preparation10% Formal Saline Preparation (Nottingham Pathology Lab) 4% Paraformaldehyde Preparation (Nottingham Pathology Lab) Fixatives (XM
Cardiomyocyte－immun...
实验概要The following protocol provides a method describing a suggested protocol for sarcomeric actinin, ANP, ER and ERb fluorescent staining of car
TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox andJosé C. Rodrig
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Immunodetection－of－cyclin－D1－and－D2/D3－using－flow－cytometry
DescriptionThis protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser ex
immunofluorescence－of－general－PBMC－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of general PBMC by Peprotech.实验步骤The following protocol used human PBMC that were
Isolation,－Culture,－Characterization－of－Cortical－and－Hippocampal－Neurons
实验概要The ability to culture primary neurons under serum-free conditions facilitates tighter control of neuronal studies. Some serum-free media and s
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1. Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2. Cells were plated on culture dishes
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers (or c