CellfreeSystemfortheexaminationofapoptoticactivity
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. The cytoplasmic extracts may be prepared from cells which were treated in culture with an apoptosis-inducing agent or from untreated cells. In the latter case, apoptotic activity can be induced by addition of apoptotic stimuli (such as recombinant active caspases or......阅读全文
Permeabilization－of－gramnegative－bacteria－with－KPi/hexane
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 oC, 200 rpm) Escherichia coli, Enterobacter aerogenes or Pseudomonas aeruginosa cells.Wash
Cell－Extraction－Protocol
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have developed
关于无细胞翻译系统的基本信息介绍
无细胞翻译系统（cell-free translation system）是2008年公布的生物化学与分子生物学名词。 没有完整细胞的体外蛋白质翻译合成系统。通常利用无细胞提取物提供所需要的核糖体、转移核糖核酸、酶类、氨基酸、能量供应系统及无机离子等，在试管中以外加的信使核糖核酸(mRNA)指
Nuclear－Extraction－Protocol
实验概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要试剂Hypotonic Buffer Solution20 mM
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials: LipofectamineBasal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aaBasal Medium containing 1% glutamineBasal Medium cont
酶活性分析（enzymatic－activity－analysis）法检定蛋白质
表现出来的酶GUS可以水解其基质衍生物pNPG,所产生的黄色生成物p-nitrophenol,可供活性测定 （Jefferson et al, 1986）；仪器用具：ELISA光度计 （Dynatech）；微量滴定盘 （microtiter plate, Nunc F96 Maxisorb 4424
流式细胞术检测细胞凋亡
实验概要Provides a rapid and convenient assay for apoptosis. 实验原理Apoptosis is a carefully regulated process of cell death that occurs as a normal part o
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－3
LDH assay LDH assay was performed in culture medium of untreated confluent cells by using a commercial kit (Sclavo Diagnostics, Siena) based on the
无细胞翻译系统的特点和应用
中文名称无细胞翻译系统英文名称cell-free translation system定 义没有完整细胞的体外蛋白质翻译合成系统。通常利用无细胞提取物提供所需要的核糖体、转移核糖核酸、酶类、氨基酸、能量供应系统及无机离子等，在试管中以外加的信使核糖核酸(mRNA)指导蛋白质的合成。常用的无细胞提取
Phospholipids－as－signalling－intermediaries
Sphingosine-1-phosphate (S1P) is an example of lipid messengers with both intracellular and extracellular functions. Intracellularly S1P regulates pro
SYSTEMGC维护保养篇－③－更换填充柱
导读在系统气相里，色谱柱是分析方案最核心的部件之一。一般情况下，色谱柱更换的频率并不高。导致色谱柱损坏或者柱效下降的原因有很多，比如载气不纯、样品污染、人为操作不当等。色谱柱本身属于消耗品，难免需要更换。看了下面视频您就可以自己动手更换色谱柱了！更换色谱柱 查看视频请点击：https://mp.we
Culture－of－human－renal－proximal－tubule－cells－and－renal－cortical－fibroblasts
1. The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2. Renal cor
AKT－Signaling－Pathway
Many cell-surface receptors induce production of second messengers like PIP3, phosphatidylinositol 3,4,5-trisphosphate, that convey signals to the cyt
DNA－laddering－assay－for－treated－cells
Characteristics of this procedure:I found the procedure described by Gong et al. to be a convenient and successful method to detect DNA laddering in c
cAMP分析－cAMP－Assays
cAMP AssaysGouzel Karimova and Daniel LadantUnite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et C
Activation－of－Src－by－Proteintyrosine－phosphatase－alpha
Progression through the cell cycle is accompanied by activation of the proto-oncogene c-Src, a protein tyrosine kinase. Overexpression of Src leads to
SCIEX临床质谱新品重磅上市－|－Citrine™－Triple－Quad™－System
SCIEX新一代液相色谱串联质谱检测系统Citrine™ Triple Quad™ System（注册证编号：国械注进20232220176）重磅发布！感谢您的一路同行，在未来，SCIEX将继续秉承“倾听客户声音，真正惠及临床”的宗旨，为广大用户持续提供高质量的产品和服务，诚挚的邀请您拨冗参加5月2
SCIEX临床质谱新品重磅上市－|－Citrine™－Triple－Quad™－System
SCIEX新一代液相色谱串联质谱检测系统Citrine™ Triple Quad™ System（注册证编号：国械注进20232220176）重磅发布！ 感谢您的一路同行，在未来，SCIEX将继续秉承“倾听客户声音，真正惠及临床”的宗旨，为广大用户持续提供高质量的产品和服务，诚挚的邀请您拨冗参
SCIEX临床质谱新品重磅上市－|－Citrine™－Triple－Quad™－System
SCIEX新一代液相色谱串联质谱检测系统Citrine™ Triple Quad™ System（注册证编号：国械注进20232220176）重磅发布！感谢您的一路同行，在未来，SCIEX将继续秉承“倾听客户声音，真正惠及临床”的宗旨，为广大用户持续提供高质量的产品和服务，诚挚的邀请您拨冗参加5月2
Dephosphorylation－...
实验概要The method provides a protocol for removal of phosphate groups from proteins, before or after blotting. To demonstrate the specificity of an ant
Skeletal－muscle－hypertrophy－is－regulated－via－AKT/mTOR－pathway
Skeletal muscle atrophies with disuse while with increased use and increased load skeletal muscle exhibits hypertrophy, with an increase in the size o
VDAC2基因突变因子与药物介绍
该基因编码电压依赖性阴离子通道成孔蛋白家族的一个成员，该家族被认为是代谢产物通过线粒体外膜扩散的主要途径编码蛋白也被认为通过调节BCL2拮抗剂/杀伤蛋白1的活性参与线粒体凋亡途径假基因已经在1号、2号、12号和21号染色体上被鉴定出来，并且在多个转录变异体中的选择性剪接结果[由RefSeq提供，20
VDAC2基因编码的功能和结构描述
该基因编码电压依赖性阴离子通道成孔蛋白家族的一个成员，该家族被认为是代谢产物通过线粒体外膜扩散的主要途径编码蛋白也被认为通过调节BCL2拮抗剂/杀伤蛋白1的活性参与线粒体凋亡途径假基因已经在1号、2号、12号和21号染色体上被鉴定出来，并且在多个转录变异体中的选择性剪接结果。This gene en
Cellulase－assays
Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p
Cellulase－assays
实验概要 Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the frui
Enzyme－Kinetics－assay－of－the－WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
A－Method－for－Structure1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
Integrin－Signaling－Pathway
Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals in response to the extracellular ma
德国IBL－ASP－ELISA试剂盒使用说明（一）
ASP (Domoic acid)direct ELISA Enzyme immunoassay for the quantitative determination of amnesicshellfish poison (Domoic acid) in algal, shellfish or se
Basic－Mechanisms－of－SUMOylation
Like ubiquitin, SUMO (small ubiquitin-related modifier) proteins are small protein tags that are conjugated to proteins to modify their function. The