MakingMetaphaseSpread
Materials:Colcemid (GIBCO: cat #: 15210-040)Hypotonic solution (pre-warmed 37C): 1:1 0.4% KCl + 0.4% Sodium citrateFixative: 3:1 MeOH + Acetic acid (e.g., 15 mL MeOH + 5 mL Acetic acid)Pasteur transpipetProcedure:Grow and harvest cells:Grow cells to 85% confluenceAdd colcemid to cell culture at 10 µl/mLIncubate for 2 hours (depends on cell cycle: longer cycl......阅读全文
Making－Metaphase－Spread
Materials:Colcemid (GIBCO: cat #: 15210-040)Hypotonic solution (pre-warmed 37C): 1:1 0.4% KCl + 0.4% Sodium citrateFixative: 3:1 MeOH + Acet
Metaphase－chromosome－preparation
Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture f
uCalpain－and－friends－in－Cell－spread
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FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
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Make DNA templates via PCR Day 1 It's preferable to start with constructs that contain RNA polymerase start sites (T3, T7, or SP6) which allow use
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2015年7月18日，由国家会议中心和雅森国际主办的“2015北京国际食品及农产品安全检测技术展览会”(China FATS Fair)在国家会议中心盛大开幕。作为国内首个以科技保障食品安全的专业展会，齐集91家行业顶尖企业参展，吸引3017名专业观众参观，展览面积达10000平方米。
CGH－Protocols－（四）
CGH Image acquisition Images were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeis
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Range ResolutionRange is the direction perpendicular to flight path of the aircraft. The vertical beamwidth , shown in Figure 3, is determined by the
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Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining2
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip. 13.
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Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
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CGH－Protocols－（一）
Metaphase chromosome preparation Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagen
Streptomyces:Protocols/Conjugation
Intergeneric Conjugation and Overlay Description Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipient
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Description Transform E.coli cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into E.coli).Approx. Duration:Prep
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Also see DNA Cloning§ Making TA Vector (Crawford Lab)T-vectors are linear-blunt-ended plasmids with a few dT's added on by Taq polymerase.
细胞遗传学——比较基因组杂交(CGH)
· Comparative Genomic Hybridization (CGH) CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are
单克隆抗体
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Transforming－chemically－competent－cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small vo
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一 教学要求 通过从土壤中分离纯化细菌 ，初步掌握微生物的分离纯化方法和无菌操作技术。 二 实验原理 - 常用的分离纯化方法 microorganisms exist in Nature as mixed populations. However, to study microo
Marcantonio－Lab－Protocol－Manual——3
Sequencing Gel Preparing and Running a Sequencing Gel (6% Polyacrylamide/Urea) A. Preparation of Gel Solution 1) Weigh out 50 g of Urea into a
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分子克隆蛋白表达实验指南（四）
7. PCR产物与TA载体连接 pGEM-T vector is T-tailed at the insert site. To improve the ligation efficiency, it is recommended PCR product be A-tailed. +A s