Protocolsforthepreparationoftumourcellsfors.c.injectionsinmice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile plastic pipettes and flasks are used. Cultures are grown in a BB6220 incubator (Heraeus) at 37 °C and 6% CO2.The tumour models used in our laboratory are obtained by subcutaneous (s.c.) injection of the following cell lines in nude mice:C51 mouse colon adenocarcinomaFE-......阅读全文
Streptomyces:Protocols/PCR
Description Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template doubl
CGH－Protocols－（四）
CGH Image acquisitionImages were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss,
CGH－Protocols－（三）
Hybridizationreagents: labeled tumor and normal-DNA (see protocol Nick translation) salmon sperm DNA, 10 mg/ml (e.g. Promega) human Cot1 DNA, 1 mg/ml
General－Cloning－Protocols
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. w
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
CGH－Protocols－（二）
DNA preparation by cryotom tissue dissectionPreparations/Materials: Cool cryostat down to -20 to -30°C about 3 hours prior to dissection Label eppendo
CGH－Protocols－（一）
Metaphase chromosome preparationMaterials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best
酵母遗传学技术
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analysis u
DNA克隆
DNA克隆（主要内容如下）· General Procedure· PCR Cloning· Subcloning· ET Cloning· Vector Preparation· Ligation Re
人源肿瘤细胞系异种移植CDX与人源肿瘤组织来源移植瘤PDX
问：什么是肿瘤移植模型？答：人的肿瘤移植到小鼠体内构建的人源化肿瘤模型问：这种模型意义在哪？答：筛药，为人民服务。（请为伟大的小鼠致敬！）问：为啥要构建肿瘤模型，不能跳过直接上临床？不也可以筛药吗？答：嗯，好主意，要不你先来好不好？.......问：什么是CDX , PDX?答：急什么，认真听小编讲
Preparation－of－Mouse－Neutrophils
实验概要Preparation of Mouse Neutrophils实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline so
specific－immunodetection－of－cyclins－using－488/630－dual－laser－flow－cytometry
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometryWilliam Telford Hospital for Special SurgeryThis protocol is f
CellTrace™－CFSE－Cell－Proliferation－Kit
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use form. The
Template－Preparation
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG preci
Platelet－Preparation
OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible! This p
Liposome－Preparation
Liposome PreparationOBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation m
CAM－preparation
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
Chemical－Induction－of－Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
人源肿瘤细胞系异种移植CDX与人源肿瘤组织来源移植瘤PDX
问：什么是肿瘤移植模型？ 答：人的肿瘤移植到小鼠体内构建的人源化肿瘤模型 问：这种模型意义在哪？ 答：筛药，为人民服务。（请为伟大的小鼠致敬！） 问：为啥要构建肿瘤模型，不能跳过直接上临床？不也可以筛药吗？ 答：嗯，好主意，要不你先来好不好？ .......
Streptomyces:Protocols/Transformation－by－Electroporation
Description Transform E.coli cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into E.coli).Approx. Duration:Prep
Rat－Blood－Collection－Protocols
实验概要The procedure presented below describes a method for collecting rat blood.实验步骤Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
ORNL－MICROARRAY－HYBRIDIZATION－PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat #
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 2
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into an adult mo
Derivation－and－Culture－of－Cortical－Astrocytes
实验概要Astrocytes are the most numerous cell type in the central nervous system (CNS). They play critical roles in adult CNS homeostasis, provide bioch
Mouse－keratinocyte－cultures
PRIMARY MOUSE KERATINOCYTE CULTURESIsolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol
Differentiate－ES－cells－into－glial－cells－and－neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1: Trypsiniz