GramStain(+\)
MaterialsColonies of bacteria from Exercise 12.2ToothpicksCrystal violetGram''s iodine95 ethanolSafraninMicroscopes with oil immersionProcedureBefore staining the individual colonies, you should first practice the technique by observation of the gram positive micro- organisms normally found in the gum linings of your mouth.Use a clean toothpick to rub along the gingival crevices (area between tooth surface an......阅读全文
Gram－Stain－(+\)
MaterialsColonies of bacteria from Exercise 12.2ToothpicksCrystal violetGram''s iodine95 ethanolSafraninMicroscopes with oil immersionProcedur
Gram－Stain－(+\)
实验概要细菌的革兰氏染色技术实验材料Colonies of bacteriaToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion实验步骤1. Before staini
细菌的简单染色(Simple－stain)与革兰氏染色(Gram－stain)
一、目的要求1、学习细菌染色的原理和方法；2、掌握细菌的简单染色法和革兰氏染色法。二、基本原理用于生物染色的染料主要有碱性染料、酸性染料和中性染料三大类。碱性染料的离子带正电荷，能和带负电荷的物质结合。因细菌蛋白质等电点较低，当它生长于中性、碱性或弱酸性的溶液中时常带负电荷，所以通常采用碱性染料（如
Testing－for－Mycoplasma－by－Indirect－DNA－Stain－(Hoechst－33258－stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
Alcian－green－stain－for－cartilage
This is an easy method for stianing cartilage that can be used with isolated or CAM-grafted limbs to study limb development or with whole embryos (8 t
Vybrant®－DyeCycle™－Ruby－stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
Human－Placental－Alkaline－Phosphatase－Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike othe
细菌检测
Gram Staining (+\-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description for Gram staining Aci
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Permeabilization－of－gramnegative－bacteria－with－KPi/hexane
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 oC, 200 rpm) Escherichia coli, Enterobacter aerogenes or Pseudomonas aeruginosa cells.Wash
革兰氏(Gram)染色液的配制方法
革兰氏(Gram)染色液 1．草酸铵结晶紫染液 A液：结晶紫(crystal violet) 2g 95%酒精 20ml B液：草酸铵(ammonium oxalate) 0．8g 蒸馏水 80ml 混合A、B二液，静置48小时后使用。 2．卢戈氏(Lugol)碘液 碘片 1．0g
细菌的鞭毛染色(Flagella－stain)
实验器材1.活材料培养12-16h的水稻黄单胞菌（Xanthomonas oryzae），粘质赛氏杆菌（Serratia marcescens）或假单细胞菌（Pseudomonas sp.）斜面菌种。2.染色液和试剂硝酸银染色液、Leifson染色液、香柏油、二甲苯。3.器材载玻片、擦镜纸、吸水纸、
细菌的荚膜染色(capsule－stain)
实验原理由于荚膜与染料间的亲和力弱，不易着色，通常采用负染色法染荚膜，即设法使菌体和背景着色而荚膜不着色，从而使荚膜在菌体周围呈一透明圈。由于荚膜的含水量在90%以上，故染色时一般不加热固定，以免荚膜皱缩变形。 实验器材 1.活材料 培养3-5天的胶质芽孢杆菌（Bacillus muci
Chromosomal－DNA－Extraction－from－Grampositive－Bacteria
Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed for Listeria monocytogenes but has worked well with othe
组织学一般技术
The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Read
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
革兰氏染色检查正常参考值及临床意义
中文名称：革兰氏染色检查 英文名称：Gram stain Examination 临床意义： 胸腹水、分泌物等涂片革兰氏染色检出细菌，可考虑为细菌感染。 对培养基生长细菌进行染色分类，并观察形态。
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
细菌的简单染色（Simple－stain）和革兰氏染色2
（3）固定：手执玻片一端，让菌膜朝上，通过火焰2-3次固定（以不烫手为宜），使细胞质凝固，以固定细菌的形态，并使其不易脱落。但不能在火焰上烤，否则细菌形态将毁坏（4）染色：将固定过的涂片放在废液缸上的搁架上，加复红染色1-2min（5）水洗：用水洗去涂片上的染色液，直至冲下之水无色时为止。（6）干燥
细菌的简单染色（Simple－stain）和革兰氏染色1
虽然各种类型的显微镜能够观察到微生物的各种形态结构，但一般实验室常用的是普通光学显微镜。由于细菌体积小且透明，在活体细胞内又含有大量的水分，因此，对光线的吸收和反射与水溶液相差不大。当把细菌悬浮在水滴内，放在显微镜下观察时，由于与周围背景没有显著的明暗差，难于看清它们的形状，更谈不上识别其细微结构。
细菌感染的微生物学检查方法
医生根据临床上诊断和治疗的需要选择不同的标本和检查方法进行实验室诊断，从而鉴定出感染的病原菌。实验室诊断包括细菌学诊断，即检测及鉴定病原菌；检测病原菌成分（抗原和核酸）以及检测患者血清中特异性抗体（图1）。图1 细菌感染的实验诊断方法 一、细菌学诊断 形态学检查 光镜下可观察直接涂片或分离培养的
革兰氏染色与细菌抗酸染色技术的操作技巧
兰氏染色和抗酸染色都属于细菌形态学检查法，提到革兰氏染色大家肯定都非常熟悉，但是说到抗酸染色你不一定了解。今天我们进入细菌的花花世界，聊一聊细菌界的这两大染色法： 革兰氏染色（Gram stain） 用途：由丹麦病理学家Christain Gram于1884年创立，直到现今，革兰氏染色法仍是细菌学
革兰氏染色反应的相关介绍
革兰氏染色反应是细菌分类和鉴定的重要性状。它是1884年由丹麦医师Gram创立的。革兰氏染色法（Gram stain）不仅能观察到细菌的形态而且还可将所有细菌区分为两大类：染色反应呈蓝紫色的称为革兰氏阳性细菌，用G+表示；染色反应呈红色(复染颜色)的称为革兰氏阴性细菌，用G-表示。 细菌对于革
革兰氏染色反应的基本内容
革兰氏染色反应是细菌分类和鉴定的重要性状。它是1884年由丹麦医师Gram创立的。革兰氏染色法(Gram stain)不仅能观察到细菌的形态而且还可将所有细菌区分为两大类：染色反应呈蓝紫色的称为革兰氏阳性细菌，用G+表示；染色反应呈红色(复染颜色)的称为革兰氏阴性细菌，用G-表示。 细菌对于革
微生物的形态观察
一．实验目的1． 学习在油镜下观察微生物 的个体形态2． 了解革兰氏染色法的原理，并熟练掌握其操作步 二．实验原理微生物的细胞小且透明，在普通光学显微镜下不易识别，必须对它们进行染色，使经染色后的菌体与背景形成明显的色差，从而能更清楚地观察到其形态和结构。因此，微生物染色技术是观察微生物形态结