LargeScalePlasmidPreps:PEGmethod
1. Grow 250 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin.2. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4°C for 10 min.3. Discard supernatant. Keep everything on ice. Resuspend bacterial pellets by adding 15 mL of water and split into 2 plastic Sorval tubes (max capacity = 40 mL/tube)4. Add 17.5 mL of fresh Solution II to each tube. Mix by inversion. Keep on ice for 10 m......阅读全文
Large－Scale－Plasmid－Preps:－PEG－method
1. Grow 250 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. 2. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm,
Large－Scale－Plasmid－Preps:－Qiagen/Cesium－Method
Most plasmids can be adequately prepped by kits containing DNA binding columns. These columns do not do a great job of separating plasmid DNA from co
Large－Scale－Plasmid,－Cosmid,－BAC,－PAC,－and－Fosmid－DNA－Isolation
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
Largescale－Immunocytology
This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes
Large－Scale－Tubulin－Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosph
酵母转化
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· Yeast Transformation (Breeden Lab)LiAc method· Large-Scale Y
Large－Scale－Tubulin－Preparation——2
III. Pouring a 1L Phosphocellulose (PC) Column Resin: Whatman P11 Cellulose Phosphate -- fibrous cation exchanger (1 gram of PC swells to about 4 m
Small－scale－DNA－preps－for－Neurospora－crassa.
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an av
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Cycle－Sequence－Reactions－For－Large－Insert－Plasmid－Templates
The following dye-labeled terminator reaction chemistries have been designed to balance conservation of reagents with the resulting sequence product s
A－Method－for－MicroScale－Isolation－and－Purification－of－Gangliosides
A Method for Micro-Scale Isolation and Purification of Gangliosides Stephan Ladisch~Director, Center for Cancer and Transplant Biology, Children'
General－Cloning－Protocols
Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a
One－step－miniprep－method－for－the－isolation－of－plasmid－DNA
plasmid miniprepAll ''miniprep'' methods reported so far for the isolation of plasmid DNA involve multiple pipetting, extraction, cent
DNA测序
DNA测序（主要内容如下）· Sequencing Gel Preparation· Preparation of Templates · DNA Sequencing by the Dideoxy Method· DNA Sequen
质粒的小量制备
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
质粒的小量制备
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
DNA转化
DNA转化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab)Very nice protocol for E. Coli transformation inc
Streptomyces:Protocols/MiniMaxi－Prep
Small Scale Plasmid Isolation (Mini / Maxi Prep)Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, normally E
BAC－DNA分离方法－Isolation－of－BAC－DNA－from－Largescale－Cultures
Isolation of BAC DNA from Large-scale Cultures Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne, Austral
质粒的Midi制备
· Plasmid Midi-preps (NWSFC)Diatomaceous Earth-based midi-prep. This procedure is the method of choice for isolating double stranded plasmid-b
细菌人工染色体
The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute) Construction of BAC
重组DNA的分离、克隆与测序实验手册7
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
PCR的下游应用
· Agarose Gel Electrophoresis of PCR Products (Robert H. Cruickshank)· Agarose Gel Electrophoresis of PCR Products (Immunology Resourc
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage . The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals pr
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
酵母转化的几种方法
Modified Yeast Transformation Inoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically, add 0.1 to 0.2 m
PCR的下游应用
・ Agarose Gel Electrophoresis of PCR Products(Robert H. Cruickshank)・ Agarose Gel Electrophoresis of PCR Products(Immunology Resource)