FluorescenceMountingMedium(Antifade)
Materials Needed20ml glass scintillation vialSmall stir barFoilGlycerol1X PBSPipets* P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730)Carbonate-Bicarbonate Buffer (see below)Wrap a glass scintillation vial with foil and drop in a small stir bar. (PPD is light-sensitive.)With a 10 ml pipet add 9 mls of glycerol to the vial.With the 1000 µl Pipetman add 1ml of 1X PBS.Place on stirrer and begin mixing.Weigh out 10 mg......阅读全文
Fluorescence－Mounting－Medium－(Antifade)
Materials Needed20ml glass scintillation vialSmall stir barFoilGlycerol1X PBSPipets* P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730)Carbonate-Bic
A－semipermanent－mounting－medium－for－immunofluorescence－microscopy
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TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
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Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
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MEDIUM－FOR－EMBRYO－RESCUE－(FOR－1－L)
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Immunofluorescence－Microscopy－Protocol
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Immunofluorescence－Microscopy－Protocol
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MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
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