FlowCellAssayswithMicrotubules:Motility／DynamicsinFluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/depolymerizing factors on microtubules. Described here are some general procedures for flow cell assays.I. Solutions & SuppliesII. VE-DIC AssaysA. MotilityB. DynamicsIII. Fluorescence AssaysA. Oxygen ScavengingB. AssaysBack to protocolsI. Solutions & SuppliesBRB......阅读全文
Flow－Cell－Assays－with－Microtubules:－Motility/Dynamics－in－Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
细胞组分和细胞器——细胞骨架
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin and Quantifying Labeling Stoi
细胞组分和细胞器——细胞器分离
Labeling Microtubules (Molecular Dynamics Inc. )Microtubules are involved in many aspects of cell motion including propulsion, mitosis, growth, and o
Vybrant®－DyeCycle™－Ruby－stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
Alexa－Fluor®－488－Annexin－V/Dead－Cell－Apoptosis－Kit
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is imp
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
线粒体荧光探针大全：TMRM，Mitotracker，JC1（4）
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
碳水化合物分析
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
Detection－Of－Cell－Viability－And/Or－Apoptosis－By－Flow－Cytometry－(FACS)
Viable cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
LIVE/DEAD®－Violet－Viability/Vitality－Kit
实验概要The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneo
实验室自动化与筛选协会2013亚洲会展短期培训课程简介
短期培训课程I ：生物制剂研发中的高通量筛选 Junma Zhou，博士，上海药明康德新药开发有限公司 刘洁颖博士，上海药明康德新药开发有限公司 In Scope of Short Course - Overview of high-throughput techniques in
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
ASENSITIVE－METHOD－FOR－DETECTION－OF－APOPTOSIS－BY－SINGLE－LASER－FLOW－CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Viscosity－－Polymeriztion－of－Microtubules
LEVEL IIMaterialsTubulin (Brain extract from Exercise 9.4)GTPATPViscometerWaterbath or incubator at 37° CProcedureCompute the amount of GTP (M.W. 523)
TEM－Visualization－of－Microtubules
LEVEL IIMaterialsCoated grid for TEM0.1 M ammonium acetate5% ethanol saturated uranyl acetateTransmission electron microscopeProcedureAt the conclusio
细胞黏附和迁移
Cell Adherence Assay (LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after the
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 µg/mL of 7-AAD. Incubatefor 20 min a
Agglutination－Assays
Agglutination AssaysREFERENCE: Lanyi, B., and T. Bergan. Methods in Microbiology, Vol 10: 93-168. BACTERIAL AGGLUTINATION: Bacterial agglutination is
Cellulase－assays
实验概要 Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the frui
Carbohydrate－Assays
Carbohydrate AssaysREFERENCE: Wright and Rebers, Anal. Biochem. 49: 307-319, 1972.OBJECTIVE: To determine the relative amounts ofLPS carbohydrates pre
Cellulase－assays
Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p
体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
Vybrant®－DyeCycle™－Green－and－Orange－Stains
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of ph
CellTrace™－CFSE－Cell－Proliferation－Kit
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use form. The
流式细胞术检测细胞凋亡
实验概要Provides a rapid and convenient assay for apoptosis. 实验原理Apoptosis is a carefully regulated process of cell death that occurs as a normal part o