FluorescenceInSituHybridizationusingTSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and was developed in collaboration with Ethan Bier and Dave Kosman at UCSD. This protocol is demonstrated with Drosophila embryos, but should be easily amenable to any specimen or tissue. TSA™ provides enzymatic fluorescent signal amplific......阅读全文
Fluorescence－Mounting－Medium－(Antifade)
Materials Needed20ml glass scintillation vialSmall stir barFoilGlycerol1X PBSPipets* P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730)Carbonate-Bic
Fluorescence－In－Situ－Hybridization－using－TSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and
ImmunohistochemistyFluorescence－Protocol2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
荧光分析法（fluorescence－analysis
荧光光谱基础； 蛋白质的荧光特性； 荧光分光光度计的结构和原理。吸收光谱和荧光光谱能级跃迁示意图 （一）荧光的产生 某些物质受紫外光或可见光照射激发后能发射出比激发光波长较长的荧光。此化学物质能从外界吸收并储存能量（如光能、化学能等）而进入激发态，当其从激发态再回复到基态时，过剩的能量可以电磁辐射的
Flow－Cell－Assays－with－Microtubules:－Motility/Dynamics－in－Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
均相荧光免疫测定（homogeneous－fluorescence－immunoassay）
均相荧光免疫测定（homogeneous fluorescence immunoassay）是根据1972年Rubenstein等建立的均相酶免疫测定法（HEI）发展形成的一种新型免疫荧光分析技术。所谓“均相”是指在反应结束后无须对游离和结合的标记物进行分离，直接测定即可。均相荧光免疫测定是利用
荧光原位杂交（Fluorescence－in－situ－hybridization,FISH）
实验原理荧光原位杂交（Fluorescence in situ hybridization FISH）是一门新兴的分子细胞遗传学技术，是20世纪80年代末期在原有的放射性原位杂交技术的基础上发展起来的一种非放射性原位杂交技术。目前这项技术已经广泛应用于动植物基因组结构研究、染色体精细结构变异分析、病
Fluorescence－Procedures－forthe－Actin－andTubulin－Cytoskeleton－in－Fixed－Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
Fluorescence－Procedures－fortheActin－andTubulin－Cytoskeleton－in－Fixed－Cells2
Formaldehyde FixationFix in 4% formaldehyde (16% stock EM grade) in CBS for 20 minutesRinse in TBSPermeabilize as for methanol fixationProcede as for
荧光原位杂交（Fluorescence－in－situ－hybridization,FISH）（图）
实验原理荧光原位杂交（Fluorescence in situ hybridization FISH）是一门新兴的分子细胞遗传学技术，是20世纪80年代末期在原有的放射性原位杂交技术的基础上发展起来的一种非放射性原位杂交技术。目前这项技术已经广泛应用于动植物基因组结构研究、染色体精细结构变异分析、病
Fluorescence－Procedures－forthe－ActinandTubulin－Cytoskeleton－inFixed－Cells1
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
荧光原位杂交（Fluorescence－in－situ－hybridization,FISH）原理
2）标本变性①将制备好的染色体玻片标本于 50oC培养箱中烤片2～3h。（经Giemsa染色的标本需预先在固定液中退色后再烤片）。②取出玻片标本，将其浸在70～75oC的体积分数70％甲酰胺/2×SSC的变性液中变性2～3min。③立即按顺序将标本经体积分数70％、体积分数90％和体积分数100％冰
Fluorescence－Procedures－forthe－ActinandTubulin－Cytoskeleton－inFixed－Cells2
Actin CytoskeletonMethanol fixationFix in -20oC methanol for 1-2.5 minutesRinse in TBSPermeabilize in TBS-0.5% TX for 10 minutesRinse in TBS-0.1% TX (
PrestoBlue™－Cell－Viability－Reagent－Protocol
实验概要PrestoBlue™ Cell Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the viability and proliferation of a wide range of cell type
alamarBlue®－Cell－Viability－Assay－Protocol
实验概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and c
AlamarBlue®－Cell－Viability－Assay
实验概要Assess cell viability. 实验原理Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activ
JC1分析线粒体膜电位的方法2
3. COMMENTARY3.1 Background information The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For th
激光显微镜的应用介绍
共聚焦及双光子在现代生物学研究中有如下应用：多色荧光成像(Multi-color imaging)，具有多磁道和双向扫描，曲线扫描等特性。三维重构（Three dimentional reconstruction）及定量分析。实时成像（Time series,real time imaging），可
激光显微镜的应用领域
共聚焦及双光子在现代生物学研究中有如下应用：多色荧光成像(Multi-color imaging)，具有多磁道和双向扫描，曲线扫描等特性。三维重构（Three dimentional reconstruction）及定量分析。实时成像（Time series,real time imaging），可
MetalEnhanced－Immu...－（一）
实验概要The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monola
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology1
By: Christopher P. Toseland1 2 , Martin R. Webb1Affiliation(s): (1) MRC National Institute for Medical Research, London, UK(2) Institut für Zelluläre
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology4
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxyATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic acid (16.4 mg,
线粒体荧光探针大全：TMRM，Mitotracker，JC1（4）
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
JIPtest和主成分分析(PCA)在植物光合作用研究中的应用4
总的来说通过PCA我们可以分类植物对各种环境因素的不同反应：(i)找到特定处理下植物样品OJIP曲线发生的特异性变化(ii)筛选出发生显著变化的JIP-test荧光参数及其变化特征，可更好对植物样品光合机构发生的变化（伤害）进行定位分析，如PSⅡ供体侧/受体测或PSⅡ活性中心等。(iii)我们还可以
DAPI－Counterstaining－Protocols
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Alexa－Fluor®－488－Annexin－V/Dead－Cell－Apoptosis－Kit
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is imp
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1. Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2. Cells were plated on culture dishes
OJIP曲线和JIPtest在植物干旱胁迫研究中的应用（三）
* 方框表示光合结构构件。绿色箭头表示可以测量的物理信号，红色箭头表示根据这些信号重新计算的电子和能量流。信号：DF，延迟荧光；PF，即时荧光；MR，调制反射；RR，远红光（735nm）反射。 * 电子流：TR，能量俘获；E21，从PSII天线到PSI的能量迁移（溢出）；ED，来自内部供
流式细胞仪的mfi－指的是什么
MFI 可以有几个意思，比如Mean Fluorescence Intensity或者Median Fluorescence Intensity。根据你样本的分布，如果样本是正态分布，平均荧光强度和中位荧光强度应该是一致的。但是在非正态分布的样本，这两个值是不一样的。具体用哪一个，要看具体样本的分布