PCRcleanup

Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or TA cloning. There is no clean up needed following genotyping reactions. PCR reactions are loaded directly on acrylamide gels.There are several ways to do the clean up:1. Run PCR product out on an agarose gel (need to use low melting point Seaplaque GTG), cut out band an......阅读全文

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