PCRcleanup
Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or TA cloning. There is no clean up needed following genotyping reactions. PCR reactions are loaded directly on acrylamide gels.There are several ways to do the clean up:1. Run PCR product out on an agarose gel (need to use low melting point Seaplaque GTG), cut out band an......阅读全文
质粒的小量制备
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
其它PCR方法
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend
Marcantonio－Lab－Protocol－Manual——3
Sequencing GelPreparing and Running a Sequencing Gel (6% Polyacrylamide/Urea) A. Preparation of Gel Solution 1) Weigh out 50 g of Urea into a clean 25
New－Digital－Conductivity－Sensors
The new four-electrode sensors have been designed to provide highly accurate measurement across a very wide range: a single UniCond 4-E measures
Removing－cells－from－liquid－nitrogen
Put cryovial straight from storage and float in the 37篊 water bath- caution should be taken as on rare occasions vials can explode when heated up due
Fastfilter－Plasmid－Midi－Kit－Vacuum/Spin－Protocol
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of
E.Z.N.A.®－Plasmid－Maxi－Kit－Spin－Protocol
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
Fastfilter－Plasmid－Midi－Kit－Spin－Protocol
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of
DNA提取纯化新技术浅谈——核酸电泳纯化技术
核酸的提取和纯化是法医DNA物证检验的关键技术之一，目前实验室最常用的方法包括核酸纯化柱（Spin column）磁珠法和clean-up系统，磁珠法由于操作简便、快速，能够提取到高纯度的核酸DNA,并且可以自动化，因此成为目前法医DNA实验室核酸提取纯化的主要手段。但磁珠法由于磁珠吸附DN
Isolation－of－Cell－Wall－Proteins－from－Medicago－sativa－Stems
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with protein
科学家争当UP主，让更多年轻人爱上科技
原文地址：http://news.sciencenet.cn/htmlnews/2023/5/501066.shtm
沃特世推出全新生物工艺walkup解决方案
美国马萨诸塞州米尔福德，即时发布–沃特世公司（纽约证券交易所代码：WAT）宣布推出全新的生物工艺walk-up解决方案，旨在进一步简化生物样品的制备和分析。该解决方案无需将生物反应器样品送至中心实验室进行分析，可显著加快上游生物工艺的开发速度，与传统方式相比可缩短多达6周的工艺开发时间。得益于沃
琼脂糖凝胶DNA纯化回收试剂盒Promega
Promega Wizard SV Gel and PCR Clean-up System 品牌：Promega回收范围：100bp-10kb价格：（50次包装的试剂盒报价为562元，11元/次）Promega的产品在进口品牌中一向以价格可亲而著称。这个胶回收试剂盒也不例外。并且这个试剂盒的性能参
Transposonmediated－Mutagenesis
Transposon-mediated MutagenesisStep 1 - Amplify ORF from MG16551.0 ul genomic DNA (30ng/ul)5.0 ul gene specific primer mix4.0
Marcantonio－Lab－Protocol－Manual——2
Quantitation of DNADetection of Nucleic Acids Using Absorption Spectroscopy The absorption of the sample can be measured at several different waveleng
Transplantation－of－theeyeforming－region－of－Amphibian－neurula－into－theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Micro
Cloning－PCR－products－using－TA－vectors
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reagents is a unique monthly column that highlights current discussions in
莱伯泰科S60UP全自动消解仪申报ANTOP奖
金秋十月，ANTOP 2018第二期申报工作正在如火如荼的进行，多家科学仪器企业竞相参与申报，这里将为您介绍ANTOP奖项“打榜”产品。 北京莱伯泰科仪器股份有限公司的S60UP全自动消解仪申请“全能消解匠心开拓者”奖。它无所不能，它可以自动加酸、自动摇匀、自动升降、自动梯度升温、自动排酸气、
Lipoprotein－Analysis－Week－2:－Electrophoresis2
Preparation of stacking gelPrepare a 7.5 ml of 3% stacking gel in a small beaker using the following amounts of appropriate reagents.Stockfinal conc.A
Phenomenex在ASMS－2009推出提高LC/MS分析速度的色谱柱
Phenomenex在ASMS 2009上介绍了用于提高实验室通量的HPLC选项，展位号为#71。海报的演讲将介绍Phenomenex的研究，包括硅胶载体柱、手性LC/MS和利用SPE萃取法净化三聚氰胺污染物。 Booth #71，用于LC/MS分析的理想色谱柱： Lux™手性
Extraction－of－25NT－RNA
实验概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation times and vo
Bioanalyzer
Protocol for Bioanalyzer RNA nano chippreparation of material12 samples per chipquantitative range 25–500 ng/μlquantitation accuracy 20%CV (for ladder
Taq酶PCR实验方法介绍
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
来自耶鲁大学的PCR常见问题的精辟总结
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINAL CON
没学过化学的化学奖得主：用微信刷淘宝，还是UP主
“我经常使用微信、支付宝、淘宝等，它们给生活带来便利。”诺贝尔化学奖得主、美国斯坦福大学教授迈克尔·莱维特，不仅演讲报告上每句话都做中文标注，并且表示，自己是“中国App爱好者”。迈克尔·莱维特做主旨报告。大湾区科学论坛供图“我很喜欢玩手机。”日前，在2023大湾区科学论坛现场接受采访时，莱维特甚至
第三届进博会临近召开－新晋“UP主”抢先看！
分析测试百科网讯 2020年初，新冠疫情爆发，在短时间内席卷全球，对各国的进出口贸易造成了沉重打击。随着中国疫情逐步缓解，国内市场成复苏之势，重新焕发出新的活力。根据商务部公布的1-8月我国进出口运行情况显示，虽然我国进出口总额20.05万亿元人民币，同比下降0.6%，但我国进出口表现也好于全球
重要转基因作物对农田生态系统的影响实验材料与方法
1)土壤理化性质及微生物数量测定 土壤微生物（细菌、真菌和放线菌）数量测定采用稀释平板法；土壤酶活性指标测定参照关松荫(1986)《土壤酶及其研究方法》的方法进行。土壤脲酶活性的测定采用苯酚钠比色法；土壤磷酸酶活性的测定采用磷酸苯二钠比色法；土壤过氧化氢酶活性的测定采用高锰酸钾滴定法；土
尿液样品净化检测硝酸盐及亚硝酸盐
J.T.Baker做为SPE(固相萃取)技术的发源地，拥有庞大的应用文献库，为了使得广大客户更好的使用SPE这项越来越被广泛应用的样品前处理技术，自2011年5月开始，J.T.Baker将定期翻译这些应用文献。 《尿液样品净化检测硝酸盐及亚硝酸盐》(Clean-up of Urine sa
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi