WIKI资讯
WIKI资讯
Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or TA cloning. There is no clean up needed following genotyping reactions. PCR reactions are loaded directly on acrylamide gels.There are several ways to do the clean up:1. Run PCR product out on an agarose gel (need to use low melting point Seaplaque GTG), cut out band an......阅读全文
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3’ Adaptor Ligation and PurificationHeat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice.Set up the 3’Adaptor ligation reaction in a
该技术在应用的过程中，肯定需要在实验条件上不断进行改进，而这种改进的好坏自然而然需要实验结果的验证。。V. Grüntzig在2002年做了该工作的一部分，结果认为，在限制性酶切时，很有必要去除其中影响DNA的酶切过程，并且实验证明了具体的酶切时间。具有说服力的结果如下： 1，
T-RFLP（末端限制性片段长度多态性）该技术在应用的过程中，肯定需要在实验条件上不断进行改进，而这种改进的好坏自然而然需要实验结果的验证。V. Grüntzig在2002年做了该工作的一部分，结果认为，在限制性酶切时，很有必要去除其中影响DNA的酶切过程，并且实验证明了具体的酶切时间。具有说服力的
Post-labeling DNA processing and purificationQiagen PCR clean up to get rid of unused oligonucleotides;Add 20µl cot1DNA, 10µl ssDNA to compete for rep
A method to re-PCR unique bands from products of mixed sizeContentsINTRODUCTIONPROTOCOLCOMMENTSINTRODUCTIONThe products of a PCR reaction - especially
INTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often contain
1B. Cloning 1. A caveat on dephosphorylation: the most common reason for failure to obtain colonies is a
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
实验概要The following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
Transposon-mediated MutagenesisStep 1 - Amplify ORF from MG16551.0 ul genomic DNA (
Load your precipitated PCR samples into 2 consecutive lanes so as not to overload the lanes. For each different sample, I would run a separate ladder
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
Labelling PCR Products with DigoxigeninPCR products may be very conveniently labelled with digoxigenin-11-dUTP (Boehringer-Mannheim) by incorpora
Labelling PCR Products with DigoxigeninPCR products may be very conveniently labelled with digoxigenin-11-dUTP (Boehringer-Mannheim) b
Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP, high-resolution IRAP/ISSR and other analyses)Saadiah Jamli
Genotyping Transgenic Rodents by PCRThis is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investig
实验概要 The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or
实验概要The iPrep™ GeneCatcher™ gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of