annealingtempfordegeneratedprimerPCRproblem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield. Formation of primer-dimer artifacts may also cause more serious problems, such as non-specific DNA synthesis (asymmetric PCR). (Help from the internet: http://www.basic.nwu.edu/biotools/oligocal...ml)Magnesium chloride is one of the main variables. It affect......阅读全文
annealing－temp－for－degenerated－primer－－PCR－problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Chemicon－试剂盒的说明
TROUBLESHOOTINGNo products are visible in any lane.1. Potential Problem: PCR amplification is not initiated.Recommendations:a. Confirm that all PCR co
sothing－about－Genome－walking
Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it
利用Mastercycler－X50系列PCR独有的2D梯度实现高效的PCR条件...
利用Mastercycler X50系列PCR独有的2D梯度实现高效的PCR条件优化Ultimate PCR Optimization with Eppendorf Mastercycler® X50 2D-gradientArora Phang, Tim Schommartz,Eppendorf
PCR－PRIMER－DESIGN－AND－REACTION－OPTIMISATION
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle NumberDenaturin
Methylation－Specific－PCR
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
PCR－Primer－Design（一）
Molecular Biology Today 2001. 2(2): 27-32. Vinay K. Singh and Anil Kumar Bioinformatics Sub-centr
PCR－Primer－Design（二）
Terminal Nucleotides Make a Difference Both the terminals of the primer are of vital importance for a successful amplification. The 3'-end
PCR－Primer－Design（三）
References Albert, J., and Fenyo, E.M. 1990. Simple, sensitive and specific detection of human immunodeficiency virus type 1 in clinical speci
Blackburn:Yeast－Colony－PCR
OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step.Updated Protocol: Blackburn Lab: Quick and Easy Yeast
Real－Time－PCR－Primer－Sets
Real Time PCR Primer SetsNOW OVER 300 PRIMER SETS!!!UPDATED: NOVEMBER 10th, 2003Quantitative RT-PCR is an important step for the validation of express
Complete－PCR－Guide
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
来自耶鲁大学的PCR常见问题的精辟总结
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINAL CON
PCR实验指导与常见问题分析3
Influence of annealing temperature and number of loci amplifiedLike any other PCR, multiplex reactions should be done at a stringent enough temperatur
Single－Primer－(SemiRandom)－PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
PCR实验指导与常见问题分析6
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
Protocol－for－dsRNA－Synthesis
实验概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
PCR
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
PCR
实验概要protocal for PCR实验步骤PCR 1) Add the following to a microfuge tube: 10 ul reaction buffer 1 ul 15 uM forward primer 1 ul 15 uM
PCR引物
PCR Primer Design and Reaction Optimization (Molecular Biology Techniques Manual) PCR Primer Design (Newman Lab) PCR Primer Design (Eppendorf)Detail
PCR引物
PCR Primer Design and Reaction Optimization (Molecular Biology Techniques Manual) PCR Primer Design (Newman Lab) PCR Primer Design (Eppendorf)Detail
Thermal－Cycling－Profile－for－Standard－PCR
Initial denaturationIt is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 94°–95°C is enou
Polymerase－Chain－Reaction－(PCR)－cont.
Polymerase Chain Reaction (PCR) cont.Choice of Polymerases for PCROne of the important advances which allowed development of PCR was the availability
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
Polymerase－Chain－Reaction－(PCR)－to－Amplify－rRNA－Gene－Fragment
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene FragmentPrepare sufficient master mix for both partners (45 mL/50 mL reaction)10 mL 10x PCR buffe
PCR实验指导与常见问题分析2
Fig. 11. Example of the influence of extension temperature. Multiplex PCR with mixtrues A-B using two different PCR programs. Reactions on the right s
PCR实验指导与常见问题分析1
CONTENTPCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using ove
Polymerase－Chain－Reaction－(PCR)－to－Amplify－rRNA－Gene－Fragment
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene FragmentPrepare sufficient master mix for both partners (45 mL/50 mL reaction)10 mL 10x PCR buffe