SinglePrimer(SemiRandom)PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources of DNA.At sufficiently low stringency, any primer will misprime while continuing to bind specifically to its intended site. Conditions can usually be found allowing mispriming sufficiently close (<3.5 kb) to the correct site to permit amplification anchored at the sa......阅读全文
Single－Primer－(SemiRandom)－PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
PCR－Primer－Design（一）
Molecular Biology Today 2001. 2(2): 27-32. Vinay K. Singh and Anil Kumar Bioinformatics Sub-centr
PCR－Primer－Design（二）
Terminal Nucleotides Make a Difference Both the terminals of the primer are of vital importance for a successful amplification. The 3'-end
PCR－Primer－Design（三）
References Albert, J., and Fenyo, E.M. 1990. Simple, sensitive and specific detection of human immunodeficiency virus type 1 in clinical speci
Real－Time－PCR－Primer－Sets
Real Time PCR Primer SetsNOW OVER 300 PRIMER SETS!!!UPDATED: NOVEMBER 10th, 2003Quantitative RT-PCR is an important step for the validation of express
PCR－PRIMER－DESIGN－AND－REACTION－OPTIMISATION
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle NumberDenaturin
Single－tube－confirmation－PCR－protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat
Single－Cell－PCR－单细胞PCR实验技术
Single Cell PCR(Protocol provided by Carolyn Troeger)Cell picking c Axiovert 100/Zeiss, extended glass capillary/Drummond, Broomall and a micromanipul
annealing－temp－for－degenerated－primer－－PCR－problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
引物设计原则（Principle－of－realtime－quantiation－PCR－primer－）
1、引物的长度一般为15-30bp，常用的是18-27bp，但不应大于38，因为过长会导致其延伸温度大于74°C，不适于Taq DNA聚合酶进行反应。2、引物序列在模板内应当没有相似性较高，尤其是3’端相似性较高的序列，否则容易导致错配。引物3’端出现3个以上的连续碱基，如GGG或CCC，也会使错误
primer－primer5怎么设计引物
首先打开软件左上角来操作file——new——dna sequence这一项可以把你复制的序列粘贴进去当然，你也可以选择另一个file——open——dna sequence去open一个新的文件。在接下来的界面里复制你的序列，于是就将序列导入了点击search进入下图界面开始设计引物在此图中有6个
Multicolour－3DFISH－in－vertebrate－cells1
Introduction Multicolour 3D-FISH in combination with confocal microscopy, 3D image reconstruction and quantitative image analysis is an efficient to
PCR实验指导与常见问题分析1
CONTENTPCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using ove
QRTPCR
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common method,
QRTPCR
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common method,
PCR实验指导与常见问题分析4
Fig. 25. Multiplex PCR of mixtures A-D comparing PCR programs with 2 (green) and 1 (yellow) minute extension time at 54° C annealing temperature. Comp
Sitedirected－Mutagenesis－using－PCR
Site-directed Mutagenesis using PCRMichael P. Weiner, Tim Gackstetter, Gina L. Costa, John C. Bauer, and Keith A. KretzFrom: Molecular Biology: Curren
Genotyping－Transgenic－Rodents－by－PCR
Genotyping Transgenic Rodents by PCR This is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigat
Long－PCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
Multiplex－PCR－Method－to－Discriminate－Artemisia－iwayomogi－from－Other－...
Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi , commonly referred to as “Haninjin,” is o
Long－PCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
TaqMan－Primer－and－Probe－Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit InstructionDesign of Probes Keep the G-C content in the 20 to 80% range.Avoid runs of an
Primer3Plus
Oligonucleotide primers are widely used in various molecular biology techniques like DNA sequencing and the polymerase chain reaction (PCR). Since a p
Primer－Premier－引物设计
Primer Premier4.0是由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计，评估的软件，和Plasmid Premier2.02一起是该公司推出的最新的软件产品。其主要界面同样也是分为序列编辑窗口（Genetank），引物设计窗口（Primer Design），
Primer－引物设计原则
概念 引物，是一小段单链DNA或RNA，作为DNA复制的起始点，在核酸合成反应时，作为每个多核苷酸链进行延伸的出发点而起作用的多核苷酸链，在引物的3′-OH上，核苷酸以二酯链形式进行合成，因此引物的3′-OH，必须是游离的。类型 存在有自然中生物的DNA复制引物（RNA引物）和聚合酶链式反应(
Streptomyces:Protocols/PCR
Description Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template dou
Streaking－Bacteria－for－Single－Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
REVERSE－TRANSCRIPTION－PCR:
REVERSE TRANSCRIPTION PCR:RNA -> LOTS OF DNAContentsReverse Transcription ReactionPolymerase Chain ReactionReverse Transcription Reaction:This provide
Chemicon－试剂盒的说明
TROUBLESHOOTINGNo products are visible in any lane.1. Potential Problem: PCR amplification is not initiated.Recommendations:a. Confirm that all PCR co
Reverse－Transcription－of－RNA
实验概要The purpose of Reverse transcription of RNA is acquiring cDNA for follow research.实验原理Reverse Transcription (RT reaction) is a process in which s