LongPCRReagentsandGuidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. PCR_protocol.html#Cheng">......阅读全文
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
LongPCR－Reagents－and－Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Long－PCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
Long－PCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
长距离PCR
· Long PCR (Church Lab)PCR conditioning for different templates, primer design, and moreLong PCR Reagents and Guidelines (Harusr/locald)Detail
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
High－Throughput－Isolation－Of－PCR－Products－Using－ChargeSwitch®－PCR－CleanUp
实验概要The ChargeSwitch® PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic aci
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
Human－Embryonic－Stem－(ES)－Cell－Protocols——Media－and－Reagents
Serum Free Media for human ES cells on MEFs: can last for 7-10 daysFinal ConcentrationAmount for 250ml Stock solution80% DMEM-F12200ml20% KO Serum Rep
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Long－Term－Storage－of－Bacterial－Strains
Purpose:Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Isolation－of－Genomic－DNA－from－Tissue－Using－ChargeSwitch®－Technology
实验概要 The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or micro (3-5 mg)
Long－Term－Storage－of－Transformed－E.coli
Long Term Storage of Transformed E.coliTransfer 10 ml of 250 ml overnight culture in sterile flip-cap 15 ml Tube.Add sterile Glycerol to 15 % final co
LongCOVID和身体不活动
不运动和COVID-19的顽固症状之间的联系正变得越来越明显。巴西圣保罗大学的研究人员最近进行的、发表在《科学报告》上的一项研究表明，那些在COVID-19中幸存下来并仍有至少一种持续症状的人，过着久坐不动的生活的可能性要大57%。此外，该研究发现，如果一个人有五个或更多的SARS-CoV-2感染的
标准PCR
· What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
标准PCR
What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for thos
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal3
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other AnalgesicsAnalgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal an
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal6
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal7
ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air
The－Impact－of－Harmo...－（二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation can
利用longread生成个人转录组
斯坦福大学医学院的遗传学教授Michael Snyder及其同事利用Pacific Biosciences系统，对三个家庭成员的类淋巴母细胞转录组进行了测序，并将获得的reads与Illumina平台上获得的较短reads进行比较。通过这些转录组，他们开发出一名家庭成员的等位基因特异的全长转录组
Method:－Preparation－of－Lymphoblastoid－Cell－Lines－for－Long－Term－Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure
Isolation－Of－PCR－Products
实验概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.实验原理The ChargeSwitch® TechnologyT