WIKI资讯
WIKI资讯
PEG PRECIPITATION OF PCR PRODUCTSThis protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing.Protocol:1. After PCR, add the following reagents to each tube:For 30 µl reaction add:4.8 µl of 5 M NaCl 4.8 µl of TE buffer 8.4 µl of 40% PEG-8000, 10mM MgCl2For 50 µl reaction a......阅读全文
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a