ThermalInactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If further manipulations of the digested DNA are to be performed, the restriction endonuclease should be inactivated. Phenol/chloroform extraction and ethanol precipitation is an irreversible method for inactivation and removal of all restriction endonucleases; however, a m......阅读全文
Thermal－Inactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If f
Serum－Thawing－－Heat－Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10º C to -40º C is stable for
Serum－Thawing－－Heat－Inactivation
How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
Thermal－Cycling－Profile－for－Standard－PCR
Initial denaturationIt is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 94°–95°C is enou
TAILPCR（thermal－asymmetric－interlaced－PCR)简介
在分子生物学研究中,基因克隆和分子杂交的探针制备等操作常需分离与已知DNA序列邻近的未知序列，TAIL-PCR又叫热不对称交错PCR，能够较好地解决上述难题。该技术通过3个嵌套的特异性引物分别和简并引物组合进行连续的PCR循环，利用不同的退火温度选择性地扩增目标片段，所获得的片段可以直接用做探针标记
Basic－handson－course－in－thermal－analysis－software－(English)
METTLER TOLEDO is pleased to announce their series of one-day training courses, which are designed to familiarize users with thermal analysis theo
Gene－Inactivation－in－the－Cyanobacterium－Synechococcus－sp.－PCC－7002－and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Const
热导检测器(thermal－conductivity－detector,TCD)结构
热敏元件装入检测池池体中，制成热导池，再将热导池与电阻组成惠斯顿电桥。
热导检测器(thermal－conductivity－detector,TCD)应用
热导检测器是一种通用的非破坏性浓度型检测器，理论上可应用于任何组分的检测，但因其灵敏度较低，故一般用于常量分析。
热导检测器(thermal－conductivity－detector,TCD)原理
热敏电阻消耗的电能所产生的热与载气热传导和强制对流等散失的热达到热动平衡，当载气中有组分进入热导池时由于组分的导热系数与载气不同，热平衡被破坏，热敏电阻温度发生变化，其电阻值也随之发生变化，惠斯顿电桥输出电压不平衡的信号，记录该信号从而得到色谱峰。
Inactivation－of－Gsk3－by－AKT－causes－accumulation－of－bcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria i
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
K通道的失活把控生物钟于白天的兴奋性
BK channel inactivation gates daytime excitability in the circadian clockJoshua P. Whitt, Jenna R. Montgomery& Andrea L. MeredithNature Communications
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（九）
The strongest and unique “acute” environment induced by cryo-thermal therapy stimulated the most effective anti-tumor immune response As described
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（九）
3) 50 mm Disks. Four 50 mm diameter, 2 mm thick disks were specially polished by Meller Optics to try to obtain strengthening disks. A previous po
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（一）
Interleukin-6 Induced Acute Phenotypic Microenvironment Promotes Th1 Anti-Tumor ImmunityTing Xue1, Ping Liu1, Yong Zhou2, Kun Liu1, Li Yang2, Robert L
膜片钳常见问题解答（二）
1. input resistance是什么意思？如何测量？ 答：在电生理学中，“input resistance”指“输入膜电阻（Rin）”。全细胞记录时，给细胞膜施加一系列刺激方波（一般为超极化），在离子通道没有开放的情况下测定跨膜电流，根据欧姆定律即可求出Rin。膜电阻（Rm）与膜输入阻
TGFTIR－(TGAFTIR)热重分析仪英文介绍
TG-FTIR (TGA-FTIR)Thermogravimetric analysis (TG) follows changes in mass of the sample as a function of temperature and/or time. TG gives charact
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（二）
Summary of ResultsThe important objective of Phase II research was achieved: to fabricate and test a prototype high power sapphire microwave windo
20点直播｜北京大学教授王玮讲述微系统热管理
直播时间：2024年9月20日（周五）20:00-21:30直播平台：科学网APPhttps://weibo.com/l/wblive/p/show/1022:2321325080680643297283（科学网微博直播间链接）科学网微博科学网视频号北京时间2024年9月20日晚八点，iCANX T
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十）
through a boundary conductance, h, on faces at z = +/- L.is controlled by the parameter hL/k, the Biot Number, where k is the thermal conductivity
利用Mastercycler－X50－PCR仪与epMotion系统实现高重复性的微...
利用Mastercycler X50 PCR仪与epMotion系统实现高重复性的微量体积PCR实验Highly reproducible low Volume PCR with Mastercycler® X50 and epMotion® Arora Phang, Tim Schommartz,
BJUT－and－H.E.L.－Joint－LaboratoryCollaborative－Excellence－for－a－Bright－Future
By the end of 2023, the number of new energy vehicles in China reached 20.4 million； whether it's power batteries or new energy vehicles, China
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（十）
In this study, we found that “acute” response was strongly induced by cryo-thermal therapy with “acute” and high expression of a series of acute p
X失活的定义
中文名称X失活英文名称X inactivation定 义雌性成体细胞中两条X染色体中的一条在遗传性状的表达上丧失功能的现象。应用学科细胞生物学（一级学科），细胞分化与发育（二级学科）
干式恒温器的简介
干式恒温器取代了传统的水浴加热方式，新型的数控金属加热方式可广泛应用于酶制剂反应，血清灭活试验，Rh血样研究，交叉配血，以及胆固醇的鉴定检测等多个领域。Instead of tradional water heating method, these newly desinged ,digital
MN1基因突变与药物因子介绍
脑膜瘤1（MN1）含有两组CAG重复序列它被脑膜瘤中的平衡易位（4；22）破坏，其失活可能参与脑膜瘤32的发病机制[由RefSeq提供，2008年7月]Meningioma 1 (MN1) contains two sets of CAG repeats. It is disrupted by a
MN1基因编码功能及结构描述
脑膜瘤1（MN1）含有两组CAG重复序列它被脑膜瘤中的平衡易位（4；22）破坏，其失活可能参与脑膜瘤32的发病机制[由RefSeq提供，2008年7月]Meningioma 1 (MN1) contains two sets of CAG repeats. It is disrupted by a
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（四）
Flow cytometrySpleens were extracted after therapy and mechanically homogenized with gentle MACS Dissociator to obtain a single-cell splenocyte su