MapKinaseInactivationofSMRTCorepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and apoptosis. Abnormal corepressor-silencer interactions have been implicated in a variety of human disease pathways including several types of leukemia. The regulation of the SMRT corepressor via the p38 and Mek-1 Kinase pathway is shown in this diagram. The EFG receptor re......阅读全文
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
Human－Cytomegalovirus－and－Map－Kinase－Pathways
To replicate in the host cell, viruses commandeer cellular signaling pathways. Cytomegalovirus (CMV) is a DNA virus with that is widespread in the pop
Regulation－of－MAP－Kinase－Pathways－Through－Dual－Specificity－Phosphatases
Mitogen-activated protein (MAP) kinases are important players in signal transduction pathways activated by a range of stimuli and mediate a number of
Thermal－Inactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If f
Phosphorylation－of－MEK1－by－cdk5/p35－down－regulates－the－MAP－kinase－pathway
Map kinases transduce responses to extracellular signals by a variety of routes, and communicate with other pathways through extensive crosstalk netwo
PTEN－dependent－cell－cycle－arrest－and－apoptosis
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
Anthrax－Toxin－Mechanism－of－Action
One of the key causes of anthrax virulence is the production of three specific factors by the gram-positive spore forming bacteria Bacillus anthracis.
Serum－Thawing－－Heat－Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10º C to -40º C is stable for
Serum－Thawing－－Heat－Inactivation
How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary or desirable to store serum
Signal－Dependent－Regulation－of－Myogenesis－by－Corepressor－MITR
The differentiation of muscle cells is regulated by many factors, including the MyoD/MEF2 family of transcription factors. The MyoD/MEF2 dimer binds t
HISTONE－KINASE－ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro
Protein－Kinase－A－at－the－Centrosome
Protein kinase A regulatory subunit RIIalpha (PKA-RIIa) is tightly bound to centrosomal structures during interphase through interaction with the A-ki
SAPK/Jun－kinase－assays
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
Sprouty－regulation－of－tyrosine－kinase－signals
Four different members of the Sprouty protein family block the cellular proliferation and differentiation induced by several different growth factors,
HDAC7基因编码功能及结构描述
组蛋白在转录调控、细胞周期进展和发育事件中起着关键作用。组蛋白乙酰化/去乙酰化改变染色体结构并影响转录因子对dna的获取。该基因编码的蛋白质与组蛋白脱乙酰酶家族成员具有序列同源性。该基因与小鼠hdac7基因同源，其蛋白通过转录辅压子smrt介导抑制作用。另外，已经发现该基因编码不同亚型的剪接转录变体
HDAC7基因突变与药物因子介绍
组蛋白在转录调控、细胞周期进展和发育事件中起着关键作用。组蛋白乙酰化/去乙酰化改变染色体结构并影响转录因子对dna的获取。该基因编码的蛋白质与组蛋白脱乙酰酶家族成员具有序列同源性。该基因与小鼠hdac7基因同源，其蛋白通过转录辅压子smrt介导抑制作用。另外，已经发现该基因编码不同亚型的剪接转录变体
HDAC7基因编码功能及结构描述
组蛋白在转录调控、细胞周期进展和发育事件中起着关键作用。组蛋白乙酰化/去乙酰化改变染色体结构并影响转录因子对dna的获取。该基因编码的蛋白质与组蛋白脱乙酰酶家族成员具有序列同源性。该基因与小鼠hdac7基因同源，其蛋白通过转录辅压子smrt介导抑制作用。另外，已经发现该基因编码不同亚型的剪接转录变体
与肺癌相关的MAP2K7基因编码功能描述
该基因编码的蛋白是一种双特异性蛋白激酶，属于MAP激酶激酶家族。这种激酶专门激活MAPK8/JNK1和MAPK9/JNK2，这种激酶本身被包括MAP3K1/MEKK1、MAP3K2/MEKK2、MAP3K3/MEKK5和MAP4K2/GCK的MAP激酶激酶激酶磷酸化并激活。这种激酶参与调节细胞对促炎
MAP2K7基因编码功能及结构描述
该基因编码的蛋白是一种双特异性蛋白激酶，属于MAP激酶激酶家族。这种激酶专门激活MAPK8/JNK1和MAPK9/JNK2，这种激酶本身被包括MAP3K1/MEKK1、MAP3K2/MEKK2、MAP3K3/MEKK5和MAP4K2/GCK的MAP激酶激酶激酶磷酸化并激活。这种激酶参与调节细胞对促炎
MAP2K7基因突变与药物因子介绍
该基因编码的蛋白是一种双特异性蛋白激酶，属于MAP激酶激酶家族。这种激酶专门激活MAPK8/JNK1和MAPK9/JNK2，这种激酶本身被包括MAP3K1/MEKK1、MAP3K2/MEKK2、MAP3K3/MEKK5和MAP4K2/GCK的MAP激酶激酶激酶磷酸化并激活。这种激酶参与调节细胞对促炎
MAP2K7与癌症相关的基因编码功能描述
该基因编码的蛋白是一种双特异性蛋白激酶，属于MAP激酶激酶家族。这种激酶专门激活MAPK8/JNK1和MAPK9/JNK2，这种激酶本身被包括MAP3K1/MEKK1、MAP3K2/MEKK2、MAP3K3/MEKK5和MAP4K2/GCK的MAP激酶激酶激酶磷酸化并激活。这种激酶参与调节细胞对促炎
SMRT测序改善CRISPR效果的评估
新兴的CRISPR技术在短短一年多的时间内掀起了基因组编辑的热潮。然而，对于这些基因改造手段的效率和结果，我们仍难以评估。目前的一些工具如荧光报告基因、克隆分析，缺乏生成报告细胞系时所需的灵敏度。高通量测序方法虽然能克服这一问题，但无奈读长太短，而供体DNA模板往往很长。 到目前为止，还没
Gene－Inactivation－in－the－Cyanobacterium－Synechococcus－sp.－PCC－7002－and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Const
MAPKinase－Signaling－Pathway
The ever evolving mitogen-activated protein kinase (MAP kinase) pathways consist of four major groupings and numerous related proteins which constitut
Peptide－map－prediction
Peptide map predictionIn identifying peptides from proteins with a known sequence, it is often useful to be able to predict how a peptide will migrate
Peptide－map－prediction
In identifying peptides from proteins with a known sequence, it is often useful to be able to predict how a peptide will migrate during electrophoresi
受体酪氨酸激酶信号通路相关MAP2K14
这个基因编码的蛋白质是MAP激酶家族的一员。MAP激酶作为多种生化信号的整合点，参与细胞增殖、分化、转录调控和发育等多种过程。这种激酶被各种环境压力和促炎细胞因子激活。活化需要MAP激酶激酶（MKKS）的磷酸化，或MAP3K7IP1/TAB1蛋白与该激酶相互作用引发的自身磷酸化。该激酶的底物包括转录
与受体酪氨酸激酶反应相关因子介绍MAPK14
这个基因编码的蛋白质是MAP激酶家族的一员。MAP激酶作为多种生化信号的整合点，参与细胞增殖、分化、转录调控和发育等多种过程。这种激酶被各种环境压力和促炎细胞因子激活。活化需要MAP激酶激酶（MKKS）的磷酸化，或MAP3K7IP1/TAB1蛋白与该激酶相互作用引发的自身磷酸化。该激酶的底物包括转录
与丝裂原活化蛋白激酶反应相关因子介绍MAPK14
这个基因编码的蛋白质是MAP激酶家族的一员。MAP激酶作为多种生化信号的整合点，参与细胞增殖、分化、转录调控和发育等多种过程。这种激酶被各种环境压力和促炎细胞因子激活。活化需要MAP激酶激酶（MKKS）的磷酸化，或MAP3K7IP1/TAB1蛋白与该激酶相互作用引发的自身磷酸化。该激酶的底物包括转录