THelperCellSurfaceMolecules
T helper cells play an essential role coordinating the activities of other parts of the immune system, including B cells, cytotoxic T cells, macrophages and other cells. The crucial nature of helper T cells in the normal immune response is demonstrated by the severe immune deficiency associated with the HIV-induced helper T cell depletion. To communicate with other cells, helper T cells express a range of cell surfac......阅读全文
T－Helper－Cell－Surface－Molecules
T helper cells play an essential role coordinating the activities of other parts of the immune system, including B cells, cytotoxic T cells, macrophag
B－Lymphocyte－Cell－Surface－Molecules
B cells must communicate with their environment to participate in the immune system as the source of antigen-specific immunoglobulins. Cell surface pr
T－Cytotoxic－Cell－Surface－Molecules
Cytotoxic T cells are a key part of the cellular immune response, killing cells that display foreign antigen on their surface, primarily virus-infecte
Monocyte－and－its－Surface－Molecules
Monocytes are a class of phagocytes involved in non-specific immune defense that develop into macrophages. In plasma, monocytes ingest and destroy bac
Neutrophil－and－Its－Surface－Molecules
Neutrophils are important phagocytotic leukocytes (white blood cells) that internalize and destroy infectious bacteria by a respiratory burst of react
Cell－Surface－Immunofluorescence－Staining－Protocol
实验概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired
Hematopoietic－Stem－Cell－Targeting－with－Surface1
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and François-Loïc CossetAdapted from Gene Transfer: Delivery
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
Dendritic－cells－in－regulating－TH1－and－TH2－Development
While T cells and B cells carry out the actions of antigen-specific immune responses, antigen-presenting cells called dendritic cells are required for
Antigen－Dependent－B－Cell－Activation
A key part of the immune system is the production of immunoglobulins (antibodies) by B cells to bind and inactivate specific foreign antigens. The bod
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
T－Cell－Receptor－and－CD3－Complex
The T cell receptor is a cell-surface receptor that recognizes specific antigen to stimulate the T cell response to that antigen. The T cell receptor
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 µg/mL of 7-AAD. Incubatefor 20 min a
HIV－Induced－T－Cell－Apoptosis
HIV infection is associated with immunosuppression caused by a dramatic reduction in the helper T cell population. The loss of helper T cells may be c
Adhesion－Molecules－on－Lymphocyte
B cell and T cell lymphocytes interact with a variety of cells as part of their immune function, circulating and homing in on specific stimuli in tiss
Integrin－Signaling－Pathway
Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals in response to the extracellular ma
Classical－Complement－Pathway
The complement system is part of the defense against invading cells and is composed of about twenty different proteins found in the plasma. When activ
Cell－to－Cell－Adhesion－Signaling
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often involving interactions
免疫荧光
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Methanol fixationForma
B－Cell－Receptor－Complex
B cells produce immunoglobulins (Ig, antibodies) that specifically bind antigen molecules. B cells first produce a membrane-bound form of immunoglobul
流式细胞仪技术专辑
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
Attenuation－of－GPCR－Signaling
The G-protein coupled receptor (GPCR) family transduces extracellular signals across the plasma membrane, activating cellular responses through a vari
Measurement－of－primary－endothelial－cell－permeability－to－fluxes－of－dextran..
Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell
Size－and－Shape－of－Protein－Molecules3
The Kirkwood/Bloomfield CalculationThe understanding of how protein shape affects hydrodynamics is elegantly extended by an analysis originally develo
Size－and－Shape－of－Protein－Molecules5
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
Size－and－Shape－of－Protein－Molecules4
Determining the Molecular Weight of a Protein Molecule—Combining S and R s à la Siegel and MonteWith the completion of multiple genomes and increasing
Size－and－Shape－of－Protein－Molecules5
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
Size－and－Shape－of－Protein－Molecules2
The sedimentation coefficient of a protein is a measure of how fast it moves through the gradient. Increasing the mass of the protein will increase it
Size－and－Shape－of－Protein－Molecules1
Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron MicroscopyAn important part of ch
流式细胞仪技术专辑
最方便的实验干货查询工具微信扫码进入「丁香实验」小程序编辑： 呜咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan