SREBPcontroloflipidsynthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol levels in the cell. When cholesterol is abundant in the cell, the SREBPs are retained in the ER. When cholesterol levels decrease, SREBPs are cleaved and released to act as transcription factors, binding to the promoters of genes such as the LDL receptor and HMG CoA Sy......阅读全文
SREBP－control－of－lipid－synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol
Regulation－And－Function－Of－ChREBP－in－Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
LIPID－EXTRACTION
PROCEDURE1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible)2. Add the same volu
Starch－Synthesis
The primary carbohydrate used for energy storage in plants is starch, the equivalent of glycogen in animals. Both starch and glycogen are polymers of
Synthesis－of－plasmalogens
Plasmalogens are a class of glycerophospholipids that contain one vinyl-ester linked long chain alcohol group in place of one ester-linked fatty acid
Sucrose－Synthesis
The prodution of sucrose takes place in plants, to store energy produced during photosynthesis. The extraction of sucrose from plants like sugar cane
Lactose－Synthesis
The synthesis of glycosidic bonds between monosaccharides requires energy input, energy that is provided by joining sugar monomers to a nucleotide suc
Pest－Control
We use Dimilin G (4% diflubenzuron) from Maag AG to fight the small dipteran black flies (Trauermücken). It is a granular substance and sprinkled
Library－cDNA－Synthesis
Library cDNA Synthesis1° cDNA SynthesisN.B: During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either
Protocol－for－dsRNA－Synthesis
实验概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
Synthesis－of－Cardiolipin－－phosphatidylinositol
Phosphoglycerides are lipids that contain glycerol with a phosphate ester on one end and two fatty acid ester side chains. Phosphoglycerides such as p
Labeled－Sphingomyelin－Synthesis
Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol.2) Allow reaction to proceed at ro
Nuclear－Receptors－in－Lipid－Metabolism－and－Toxicity
Nuclear receptors are transcription factors that are activated upon binding to its ligands. Initially, they had been classified as classic endocrine n
De－novo－synthesis－of－pyrimidine－nucleotides
NON-Clickable Image The pyrimidine ribonucleotides, those found in RNA, are uracil and cytosine, each containing the sugar ribose and a nitrogenous ba
Universal－RiboClone®－cDNA－Synthesis－System
Universal RiboClone® cDNA Synthesis SystemThe Universal RiboClone® cDNA Synthesis System contains the reagents required for the synthesis of double-st
Culture－of－Endometrial－Explants－to－Monitor－Synthesis
Reprinted from:Hansen, P.J and Betts, J.G. (1992) Culture of endometrial explants and peri-implantation conceptuses to monitor synthesis and secretion
Lipid－analysis：EXTRACTION－OF－LIPIDS－FROM－LIPOPHORIN
Lipid analysisEXTRACTION OF LIPIDS FROM LIPOPHORIN Since lipids are hydrophobic, they are better soluble in organic solvents than in water. Because th
筛分仪－AS－300－control特点
德国RETSCH（莱驰）的AS300control分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。AS 300 control 机型是专门为直径为305毫米（12英寸）的分析筛而设计的。与直径为20
International－Process－Analytics－and－Control－Congress
International Process Analysis and Control Congress, China 2012IPAC 2012 Call for paper and Introduction The International Process Analysis & Control
Industry－Update－－Uptime－and－Control－in－Petrochemicals
The requirements for petrochemicals and related products are continuously updated and adapted to changes in the industry. The challenge for prof
筛分仪－AS－200－control特点
德国RETSCH（莱驰）的AS200系列分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。所有AS 200 系列机型均采用电磁驱动动力。这是RETSCH 的一项ZL技术（EP 0642844）。这
筛分仪－AS－400－control特点
AS400control分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。该机型应用于干筛，其所使用的分析筛的直径最大可达到400 毫米。此机型所产生的均一的、水平转动式的筛分运动使筛分物能够得以精确地分离。对于十分注重精确度和操作舒适性的用户，AS400系列的全
Cadmium－induces－DNA－synthesis－and－proliferation－in－macrophages
Exposure to divalent cadmium ions (Cd2+) is a known cancer risk factor, but the molecular mechanisms responsible for the inappropriate induction of ce
DNA合成（DNA－synthesis－）技术介绍
蛋白质概念提出：DNA多肽合成（DNA synthesis ）：按照预定核苷酸的顺序，将脱氧核苷酸逐个进行人工连接合成DNA链的方法。目前多是采用固相合成法，即是在多聚体支持物上从3′端延伸核苷酸，可自动化操作。)目前，oligo DNA合成一般都采用固相亚磷酰胺三酯法合成DNA片段，此方法具有高效
内质网的EOR和SREBP反应介绍
内质网超负荷反应（EOR） 细胞除了用UPR应对错误蛋白过度堆积于内质网，还会启动其他机制来应对紧急情况，比如激活细胞核因子NF-kB来引发EOR反应，最终产生对前炎性细胞因子，进而激活细胞存活、凋亡、细胞炎症反应和细胞分化等相关信号途径。 胆固醇调节级联反应（SREBP） 该反应主要涉及
Determination－and－Detection－of－Reactive－Oxygen－Species－(ROS),－Lipid－...
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate variou
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY
During this week, you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a
Proepithelin－Conversion－to－Epithelin－and－Wound－Repair－Control
This diagram illustrates the opposing effects that Proepithelin (PEPI) and the elastase digest fragments, the epithelins (EPIa-g), have on epithelial
Control－of－Gene－Expression－by－Vitamin－D－Receptor
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti