Proteinpurification;actin

Protein－purification;－actin

Protein purification; actin      Overview   ACTINThe most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various

Actin－Capture－Assay

David AmbergDialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 .Mix 5ug actin into 50ul total volume binding buffer.Mix 5ug GST-fusio

大鼠Actinb(Actinb)ELISA检测法

大鼠Actin-b(Actin-b)ELISA试剂盒 (用于血清、血浆、细胞培养上清液和其它生物体液内) 原理本实验采用双抗体夹心 ABC-ELISA法。用抗大鼠 Actin-b 单抗包被于酶标板上，标准品和样品中的 Actin-b与单抗结合，加入生物素化的抗大鼠Actin-b，形成免疫复合物连接在

Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose

实验概要This  protocol is designed as a quick purification method for antibodies from  mammalian sera, ascites, and cell culture supernatants主要试剂 Protein

Antibody－Purification－using－Protein－A,－Protein－G,－or－Protein－L－Agarose

实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should

Actin－StainingActin－Staining－Protocol

实验概要Invitrogen  offers several fluorescent and biotinylated phalloidin and phallacidin  derivatives for labeling F-actin. These phallotoxins, isolated

Isolation－of－Actin－and－Myosin－Filaments

LEVEL IIIMaterialsRelaxing Solution0.1 M KCl0.001 M MgCl5 mM ATP0.016 M NaHPONaHPOAdjust pH to 7.30.05 M Sodium phosphate buffer, pH 7.00.001 M EDTA (

Y－branching－of－actin－filaments

βActin抗体推荐—Abbkine高效价的BetaActin内参抗体

Actin即肌动蛋白，是细胞的一种重要骨架蛋白。Actin大致可分为六种，其中四种是不同肌肉组织特异性的，其余两种广泛分布于各种组织中，包括 β-actin和γ-non-muscle actin。β-actin作为内参是得到了公认的，这是针对大多数组织和细胞来说的，它广泛分布于细胞浆内，表达

Isolation－of－Nonmuscle－Actin

General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buffer G and dilute as needed from frozen aliquots of 50X Buffer G.1X Extr

常用的βactin－引物序列

human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit act

常用的βactin－引物序列

human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit act

大鼠Actinb(Actinb)ELISA试剂盒使用说明

Protein－Electrophoresis

DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh

Protein－Crystallization

Background:Proteins, like many molecules, can be prompted to form crystals when placed in the appropriate conditions. In order to crystallize a protei

Radioiodination－of－protein

Radioiodination (by Jun Takagi,6/16/2000)Purpose and backgroundsPrinciple of radioiodinationAddition of oxidizing reagents (such as chloramine-T or pe

The－Determination－of－Proteinprotein－Interactions－by－the－Matingbased－...

Dynamic and reversible protein–protein interactions have a pivotal function in all living cells. For instance, protein–protein interactions are in

Preparation－of－Conventional－Actin－from－Skeletal－Muscle

Modified from Spudich & Watt, 1971, JBC 246:4866.1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min.

Eukaryotic－protein－translation

The scanning translation initiation model suggests that 40S ribosomal subunit preloaded with factors bind to the 5’ end of the mRNA near the cap. The

Protein－Assay－(Spectrophotometer)

Protein Assay (Spectrophotometer)Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve.Place 0, 2, 5, 10, 15,

Acetone－precipitation－of－protein

This procedure is suitable for recovering proteins from most aqueous solvents and from SDS containing buffers. It is not recommended for proteins diss

Lowry－–－Protein－Determination

Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat

LOWRY－PROTEIN－ASSAY

The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-

Protein－Kinase－A－at－the－Centrosome

Protein kinase A regulatory subunit RIIalpha (PKA-RIIa) is tightly bound to centrosomal structures during interphase through interaction with the A-ki

Preparing－a－Selenomethionyl－Protein

PurposeThe protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determinat

Bradford－protein－assay

Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly

Protein－Staining－Procedures

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor

BIURET－PROTEIN－ASSAY

BIURET PROTEIN ASSAYMATERIALSBiuret ReagentBovine serum albumin (BSA)Spectrophotometer and tubesPROCEDUREPrepare standard dilutions of BSA containing

Protein－A－Purification－of－Antibody

1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buff

Bradford－–－Protein－Determination

Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster