PulseFieldElectrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established technique by the early 1970's.Typically, DNA was isolated intact and then treated with restriction enzymes to generate pieces small enough to resolve by electrophoresis in agarose or acrylamide. Although this procedur......阅读全文
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Dark－Field－Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
脉冲[交变]电场凝胶电泳的技术介绍
中文名称脉冲[交变]电场凝胶电泳英文名称pulse [alternative] field gel electrophoresis定 义利用脉冲电场的作用在凝胶介质中分离大分子DNA的一种电泳方法。由于超过一定大小（大于40 kb）的线状DNA分子在琼脂糖凝胶中电泳的速度几乎相同，无法在恒场强凝胶
脉冲[交变]电场凝胶电泳的定义
中文名称脉冲[交变]电场凝胶电泳英文名称pulse [alternative] field gel electrophoresis定 义利用脉冲电场的作用在凝胶介质中分离大分子DNA的一种电泳方法。由于超过一定大小（大于40 kb）的线状DNA分子在琼脂糖凝胶中电泳的速度几乎相同，无法在恒场强凝胶
脉冲[交变]电场凝胶电泳
中文名称脉冲[交变]电场凝胶电泳英文名称pulse [alternative] field gel electrophoresis定 义利用脉冲电场的作用在凝胶介质中分离大分子DNA的一种电泳方法。由于超过一定大小（大于40 kb）的线状DNA分子在琼脂糖凝胶中电泳的速度几乎相同，无法在恒场强凝胶
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
PURELAB－Pulse的EDI技术
连续电去离子技术连续电去离子技术（EDI，Electrodeionization）是一种融合了电渗析技术，离子交换（IX）技术和阳、阴离子选择透过膜的先进绿色环保的水处理技术。经过反渗透膜处理后的纯水进入EDI模块。在电场的作用下，水中离子在树脂中定向迁移，通过阳、阴离子选择透过膜。这些离子集中到浓
Construction－of－BAC－Libraries：Construction－of－a－BAC－library
Once high molecular weight (HMW) DNA has been prepared it must somehow be fragmented and DNA in the desired size range isolated. In general, as the de
Chromatin－Electrophoresis
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus CollegeExercise 10.4 - Chromatin ElectrophoresisLEVEL IIMaterials 14 M Urea6 M NaCl0.05
RNA－Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
electrophoresis－of－DNA
Agarose Gel Electroporesis of DNA Making the gel: 1. Place casting platform with well former sideways in gel stand where you wish to pour
DNA－Electrophoresis
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively
Capillary－Electrophoresis
Capillary electrophoresis is a very sensitive analytical technique. Sample components are separated within a fused silica capillary using one of sever
Protein－Electrophoresis
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
旷场实验－(open－field－test)
实验概要 观察实验动物在新异环境中的自主行为、探究行为与紧张度。实验原理 旷场（open field test）分析系统是观察研究实验动物神经精神变化、进入开阔环境后的各种行为，例如动物对新开阔环境的恐惧而主要在周边区域活动，在中央区域活动较少，但动物的探究特性又促使其产生在中央区域活动的动机，也可
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
旷场实验－(open－field－test)
实验概要观察实验动物在新异环境中的自主行为、探究行为与紧张度。实验原理旷场（open field test）分析系统是观察研究实验动物神经精神变化、进入开阔环境后的各种行为，例如动物对新开阔环境的恐惧而主要在周边区域活动，在中央区域活动较少，但动物的探究特性又促使其产生在中央区域活动的动机，也可
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
Agarose－Gel－Electrophoresis
实验概要Separating nucleic acid fragments by agarose gel electrophoresis.实验原理 Agarose gel electrophoresis remains the most widely used technique for sep
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
SonTek/YSI开发出Smart－Pulse－HD－技术
YSI旗下子公司--世界上声学多普勒技术的领导者之一的SonTek/YSI,日前推出了集当前所有成熟的多普勒技术测流为一身的Smart Pulse HD 技术。 该技术以一年多前开发出的新产品RiverSurveyor S5/M9为平台，具备了如它的名字所述的应用优势
Protocol－for－Dual－Pulse－Labeling－Using－EdU－and－BrdU－Incorporation
实验概要The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation is traditi
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p
Blue－Native－Gel－Electrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m
Standard－neutral－agarose－electrophoresis
Standard neutral agarose electrophoresisStandard agarose gels can be prepared using either TBE or TAE running buffers.You will need:Either 10 x TBE or